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Related Experiment Video

Updated: Jun 3, 2026

Following Cell-fate in E. coli After Infection by Phage Lambda

Following Cell-fate in E. coli After Infection by Phage Lambda

Published on: October 14, 2011

Molecular cloning in bacteriophage lambda and in cosmids.

C Christiansen¹

¹Gensplejsningsgruppen, The Technical University of Denmark, Lyngby, Denmark.

Methods in Molecular Biology (Clifton, N.J.)

|March 5, 2011

Summary

Bacteriophage lambda has a double-stranded DNA genome that is linear within the phage but circularizes upon infection. This circularization is mediated by complementary DNA ends known as the cos region.

Area of Science:

Molecular Biology
Virology
Genetics

Background:

Bacteriophage lambda is a well-studied virus that infects bacteria.
Understanding viral DNA structure and replication is crucial in molecular biology.

Purpose of the Study:

To describe the structure of the Bacteriophage lambda DNA molecule.
To explain the initial step of Bacteriophage lambda infection.

Main Methods:

Analysis of Bacteriophage lambda DNA structure.
Description of DNA end complementarity.

Main Results:

Bacteriophage lambda possesses a double-stranded DNA genome of approximately 50 kilobases.
The DNA molecule is linear in the phage particle.

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The DNA has single-stranded complementary ends (cos region).

Conclusions:

The cos region facilitates the joining of linear DNA ends to form a circular molecule.
Circularization is the first step in the Bacteriophage lambda infection process.