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Updated: Jun 3, 2026
06:14
Growth, Purification, and Titration of Oncolytic Herpes Simplex Virus
Published on: May 13, 2021
HSV Growth, Preparation, and Assay
1Institute of Neurological Sciences, Southern General Hospital, Neurovirology Research Laboratories, University of Glasgow, Scotland, UK.
Methods in Molecular Medicine
|March 5, 2011
Summary
This chapter details essential techniques for growing and assaying herpes simplex virus (HSV). Proper methods ensure contamination-free, high-titer virus stocks for reliable experimental results.
Area of Science:
- Virology
- Molecular Biology
- Eukaryotic Systems
Background:
- Herpes simplex virus (HSV) research requires reliable virus stocks.
- Experimental work with HSV necessitates proper cultivation and assaying.
Purpose of the Study:
- To provide fundamental techniques for growing and assaying HSV.
- To address the common lack of understanding in achieving high-quality virus stocks.
Main Methods:
- Cultivation of HSV under controlled conditions.
- Assaying virus titers and purity.
- Achieving low particle to plaque-forming units (PFU) ratio.
Main Results:
- Detailed protocols for contamination-free virus stock preparation.
- Methods for obtaining high-titer HSV stocks.
- Strategies for ensuring genetic purity of virus stocks.
Conclusions:
- Mastering HSV cultivation and assaying is a critical first step in virology research.
- Understanding basic requirements leads to more reliable and reproducible experimental outcomes.
- High-quality virus stocks are essential for both pathogen studies and as model eukaryotic systems.
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