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Genital herpes is a sexually transmitted infection primarily caused by herpes simplex virus type 2 (HSV-2), though herpes simplex virus type 1 (HSV-1) is increasingly implicated in genital infections, particularly among younger populations. Transmission occurs mainly through sexual contact, with asymptomatic viral shedding serving as a major route of spread. This characteristic makes HSV-2 difficult to control at a population level, as individuals may unknowingly transmit the virus even in the...

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Updated: Jun 3, 2026

Growth, Purification, and Titration of Oncolytic Herpes Simplex Virus

Growth, Purification, and Titration of Oncolytic Herpes Simplex Virus

Published on: May 13, 2021

HSV Growth, Preparation, and Assay.

J Harland¹, S M Brown

¹Institute of Neurological Sciences, Southern General Hospital, Neurovirology Research Laboratories, University of Glasgow, Scotland, UK.

Methods in Molecular Medicine

|March 5, 2011

Summary

This chapter details essential techniques for growing and assaying herpes simplex virus (HSV). Proper methods ensure contamination-free, high-titer virus stocks for reliable experimental results.

Area of Science:

Virology
Molecular Biology
Eukaryotic Systems

Background:

Herpes simplex virus (HSV) research requires reliable virus stocks.
Experimental work with HSV necessitates proper cultivation and assaying.

Purpose of the Study:

To provide fundamental techniques for growing and assaying HSV.
To address the common lack of understanding in achieving high-quality virus stocks.

Main Methods:

Cultivation of HSV under controlled conditions.
Assaying virus titers and purity.
Achieving low particle to plaque-forming units (PFU) ratio.

Main Results:

Detailed protocols for contamination-free virus stock preparation.

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Last Updated: Jun 3, 2026

Growth, Purification, and Titration of Oncolytic Herpes Simplex Virus

Growth, Purification, and Titration of Oncolytic Herpes Simplex Virus

Published on: May 13, 2021

Plaquing of Herpes Simplex Viruses

Plaquing of Herpes Simplex Viruses

Published on: November 5, 2021

Determination of Biofilm Initiation on Virus-infected Cells by Bacteria and Fungi

Determination of Biofilm Initiation on Virus-infected Cells by Bacteria and Fungi

Published on: July 6, 2016

Methods for obtaining high-titer HSV stocks.

Strategies for ensuring genetic purity of virus stocks.

Conclusions:

Mastering HSV cultivation and assaying is a critical first step in virology research.
Understanding basic requirements leads to more reliable and reproducible experimental outcomes.
High-quality virus stocks are essential for both pathogen studies and as model eukaryotic systems.