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Updated: Jun 3, 2026
11:56
Ex Vivo Infection of Murine Epidermis with Herpes Simplex Virus Type 1
Published on: August 24, 2015
HSV Entry and Spread
1Institute of Neurological Sciences, Southern General Hospital NHS Trust, University Department of Neurology, Glasgow, Scotland, UK.
Methods in Molecular Medicine
|March 5, 2011
Summary
This chapter details assays for tracking herpes simplex virus type 1 (HSV-1) cell entry and spread. It highlights key viral glycoproteins like gB, gC, and gD involved in cell attachment and fusion.
Area of Science:
- Virology
- Cell Biology
- Molecular Biology
Background:
- Herpes simplex virus type 1 (HSV-1) entry and cell-to-cell spread are intricate processes.
- These processes involve multiple HSV-encoded membrane proteins, including glycoproteins.
Purpose of the Study:
- To describe assays used to monitor HSV-1 entry and spread in cell culture.
- To review the roles of specific HSV-encoded glycoproteins in viral entry.
Main Methods:
- The chapter focuses on commonly used assays for observing HSV-1 cell culture dynamics.
- It discusses the functions of viral glycoproteins in cell recognition, attachment, and fusion.
Main Results:
- HSV-1 entry involves initial attachment mediated by glycoproteins gC and gB interacting with heparan sulfate proteoglycans.
- A more stable attachment is mediated by gD, followed by membrane fusion requiring gB and gH/gL.
Conclusions:
- Specific viral glycoproteins (gB, gC, gD, gH/gL) play crucial roles in HSV-1 entry.
- The precise functions of some proteins and the roles of other membrane proteins in entry into different cell types require further investigation.
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