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Related Concept Videos

Gene Therapy00:59

Gene Therapy

Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be inserted. The...
Gene Therapy00:59

Gene Therapy

Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be inserted. The...
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Herpes

Herpes simplex type 1 (HSV‑1) is a widespread pathogen responsible for orolabial lesions. It is an enveloped, double-stranded DNA (dsDNA) virus belonging to the family Herpesviridae. Once the virus infects a host cell, its double‑stranded DNA genome is delivered into the nucleus, where a coordinated cascade of immediate‑early, early, and late gene expression directs viral DNA replication, structural protein synthesis, and virion assembly. After primary infection of epithelial cells, HSV-1...
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Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...
Genital Herpes01:23

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Genital herpes is a sexually transmitted infection primarily caused by herpes simplex virus type 2 (HSV-2), though herpes simplex virus type 1 (HSV-1) is increasingly implicated in genital infections, particularly among younger populations. Transmission occurs mainly through sexual contact, with asymptomatic viral shedding serving as a major route of spread. This characteristic makes HSV-2 difficult to control at a population level, as individuals may unknowingly transmit the virus even in the...

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Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing
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HSV Vectors for Gene Therapy.

D C Bloom1

  • 1Department of Microbiology and Immunology, Reed Neurological Research Centre, UCLA Medical Centre, Los Angeles, CA.

Methods in Molecular Medicine
|March 5, 2011
PubMed
Summary
This summary is machine-generated.

Herpes simplex virus (HSV) is a promising vector for nervous system gene expression. Its ability to establish lifelong latent infections in neurons and efficiently carry foreign genes makes it ideal for neuroscience research.

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Area of Science:

  • Neuroscience
  • Virology
  • Gene Therapy

Background:

  • Herpes simplex virus (HSV) possesses biological characteristics suitable for gene delivery vectors.
  • The nervous system presents unique challenges and opportunities for gene therapy applications.

Purpose of the Study:

  • To evaluate the potential of herpes simplex virus (HSV) as a vector for expressing foreign genes within the nervous system.
  • To highlight the advantages of using HSV for neural gene delivery.

Main Methods:

  • Review of existing literature on herpes simplex virus biology and its application as a vector.
  • Analysis of HSV's natural infection patterns in neuronal cells.

Main Results:

  • HSV establishes lifelong latent infections in both peripheral and central nervous system neurons.
  • Latent HSV genomes exist as episomal copies within neurons, with no known integration.
  • Nonreplicating HSV recombinants efficiently establish latent infections.

Conclusions:

  • Herpes simplex virus (HSV) is an attractive vector for nervous system gene expression due to its ability to establish persistent, non-integrating latent infections in neurons.
  • The efficient latency establishment by nonreplicating HSV recombinants further supports its utility.
  • HSV vectors offer a viable tool for long-term gene expression in neural circuits.