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Isolation and Adoptive Transfer of High Salt Treated Antigen-presenting Dendritic Cells
Published on: March 5, 2019
Isolation of mouse spleen dendritic cells.
1Antigen Presentation Research Group, Imperial College School of Medicine, Northwick Park Hospital, London, UK.
Methods in Molecular Medicine
|March 5, 2011
Summary
Dendritic cells (DC) are crucial immune cells. While researchers can grow DC from bone marrow, their relationship to DC found naturally within tissues remains unclear, necessitating further study.
Area of Science:
- Immunology
- Cell Biology
Background:
- Dendritic cells (DC) were first identified over 20 years ago.
- DC are a small but significant proportion of spleen cells, allowing for purification.
- In vitro culture methods now allow for the generation of DC from bone marrow progenitors.
Purpose of the Study:
- To investigate the relationship between in vitro cultured dendritic cells (DC) and those found in vivo.
- To highlight the ongoing need for research into tissue-resident DC populations.
Main Methods:
- Isolation and purification of DC from mouse spleens.
- In vitro culture of bone marrow progenitors to generate DC-like cells.
- Phenotypic and functional analysis of both purified and cultured cells (implied).
Main Results:
- Spleen is a viable source for purifying functional dendritic cells (DC).
- Bone marrow progenitor cultures yield cells with DC characteristics.
- The in vivo relevance of cultured DC remains to be determined.
Conclusions:
- Despite advancements in in vitro DC generation, studying naturally occurring DC in tissues is essential.
- Further research is required to understand the in vivo identity and function of DC populations.
