Murine intestinal dendritic cell isolation
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Updated: Jun 3, 2026
Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine
Published on: May 21, 2012
1Gastroenterology, The Canberra Hospital, Australia.
This chapter describes techniques for the isolation of dendritic cell-enriched single-cell suspensions from the lamina propria (LP) of both small and large intestine and from Peyer's patches (PP). This technique can also be used to obtain intestinal macrophage-enriched populations. The characterization of an assay system, the allogeneic mixed leukocyte reaction (MLR), is also described.
This chapter describes techniques for the isolation of dendritic cell-enriched single-cell suspensions from the lamina propria (LP) of both small and large intestine and from Peyer's patches (PP). This technique can also be used to obtain intestinal macrophage-enriched populations. The characterization of an assay system, the allogeneic mixed leukocyte reaction (MLR), is also described.
