Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 3, 2026

Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine
09:25

Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine

Published on: May 21, 2012

Murine intestinal dendritic cell isolation.

P Pavli1

  • 1Gastroenterology, The Canberra Hospital, Australia.

Methods in Molecular Medicine
|March 5, 2011
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Safe and effective: anti-tumour necrosis factor therapy use in pregnant patients with Crohn disease and ulcerative colitis.

Internal medicine journal·2016
Same author

Placental involvement by non-Hodgkin lymphoma in a Crohn disease patient on long-term thiopurine therapy.

Internal medicine journal·2016
Same author

Spondyloarthropathy in inflammatory bowel disease patients on TNF inhibitors.

Internal medicine journal·2015
Same author

Infliximab reverses inflammatory muscle wasting (sarcopenia) in Crohn's disease.

Alimentary pharmacology & therapeutics·2015
Same author

Hepatosplenic T-cell lymphoma, immunosuppressive agents and biologicals: what are the risks?

Internal medicine journal·2014
Same author

Early predictors of colectomy and long-term maintenance of remission in ulcerative colitis patients treated using anti-tumour necrosis factor therapy.

Internal medicine journal·2014
Same journal

Erratum to: Immunotherapeutic Approach to Cancer with Cutaneous DNA Vaccination.

Methods in molecular medicine·2015
Same journal

Methods for cancer gene therapy using tumor suppressor genes.

Methods in molecular medicine·2014
Same journal

Suppression of the human carcinoma phenotype by an antioncogene ribozyme.

Methods in molecular medicine·2014
Same journal

Methods for the use of stromal cells for therapeutic gene therapy.

Methods in molecular medicine·2014
Same journal

Methods for adenovirus-mediated gene transfer to synovium in vivo.

Methods in molecular medicine·2014
Same journal

Methods for gene transfer to synovium.

Methods in molecular medicine·2014
See all related articles

This study details methods for isolating dendritic cells and macrophages from the gut lamina propria and Peyer's patches. These techniques also enable the characterization of the allogeneic mixed leukocyte reaction assay system.

Area of Science:

  • Immunology
  • Gastroenterology
  • Cell Biology

Background:

  • Dendritic cells (DCs) and macrophages are crucial immune cells residing in the intestinal lamina propria (LP) and Peyer's patches (PP).
  • Efficient isolation of viable immune cells from these tissues is essential for studying intestinal immunity.
  • Existing methods may not optimally yield enriched populations of specific cell types for downstream analysis.

Purpose of the Study:

  • To describe robust techniques for isolating dendritic cell-enriched and macrophage-enriched single-cell suspensions from the intestinal LP and PP.
  • To provide a method for characterizing an allogeneic mixed leukocyte reaction (MLR) assay system.

Main Methods:

  • Single-cell suspensions were prepared from the lamina propria of the small and large intestine, as well as from Peyer's patches.

More Related Videos

Isolation of Mouse Lung Dendritic Cells
09:04

Isolation of Mouse Lung Dendritic Cells

Published on: November 22, 2011

An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets
09:09

An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets

Published on: April 18, 2016

Related Experiment Videos

Last Updated: Jun 3, 2026

Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine
09:25

Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine

Published on: May 21, 2012

Isolation of Mouse Lung Dendritic Cells
09:04

Isolation of Mouse Lung Dendritic Cells

Published on: November 22, 2011

An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets
09:09

An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets

Published on: April 18, 2016

  • Enrichment strategies were employed to obtain populations enriched for dendritic cells and macrophages.
  • The allogeneic mixed leukocyte reaction (MLR) was established and characterized as an assay system.

    • Main Results:

    • The described techniques successfully yielded dendritic cell-enriched single-cell suspensions from both small and large intestinal LP and PP.
    • Intestinal macrophage-enriched populations were also obtainable using these methods.
    • The allogeneic MLR assay system was characterized, providing a tool for functional immune studies.

    Conclusions:

    • The presented chapter offers reliable protocols for isolating key immune cell populations from the gut.
    • These methods facilitate further research into intestinal immune responses and cell-cell interactions.
    • The characterized MLR assay system serves as a valuable tool for evaluating T-cell activation in the context of intestinal immunity.