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Human intestinal dendritic cell isolation.

P Pavli1

  • 1Gastroenterology, The Canberra Hospital, Australia.

Methods in Molecular Medicine
|March 5, 2011
PubMed
Summary
This summary is machine-generated.

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Veiled cells, identified in inflammatory bowel disease patients, are more numerous in inflamed intestines. Isolating human dendritic cells (DC) presents challenges due to low numbers and lack of specific markers.

Area of Science:

  • Immunology
  • Gastroenterology
  • Cell Biology

Background:

  • Veiled cells, resembling dendritic cells (DC), were first observed in the human intestinal lamina propria of inflammatory bowel disease (IBD) patients.
  • These cells were found in higher quantities in inflamed intestinal tissue compared to healthy controls.
  • The isolation of human intestinal dendritic cells (DC) is challenging due to low cell numbers and a scarcity of specific surface markers.

Purpose of the Study:

  • To highlight the challenges in isolating dendritic cells (DC) from human intestinal tissue.
  • To discuss the specific difficulties encountered when working with human tissues for DC isolation.
  • To present advanced techniques for obtaining pure populations of human DC.

Main Methods:

  • Morphological identification of veiled cells in human intestinal lamina propria.

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  • Comparison of cell numbers in inflamed versus normal intestinal tissues.
  • Discussion of techniques for isolating dendritic cells (DC) from human tissues, including fibronectin adherence and cell sorting via negative selection with monoclonal antibodies.

    • Main Results:

    • Veiled cells were observed in human intestinal lamina propria, particularly in patients with inflammatory bowel diseases.
    • Increased numbers of these cells were noted in inflamed bowel tissue compared to normal controls.
    • Standard isolation methods are complicated by properties like fibronectin adherence and complement sensitivity, necessitating sophisticated techniques like negative selection cell sorting.

    Conclusions:

    • Human intestinal dendritic cell (DC) isolation is complex, requiring advanced methods.
    • Properties such as fibronectin adherence and complement sensitivity pose significant hurdles.
    • Sophisticated techniques, including cell sorting, are essential for obtaining pure human DC populations.