In vivo assays of langerhans cell migration

The skin is an immunologically active tissue; the integrated skin immune system comprises epidermal Langerhans cells (LC), dermal dendritic cells (DC), tissue macrophages, mast cells, and T lymphocytes in transit. Cutaneous immune function is orchestrated through the action of cytokines produced locally in the skin by LC, keratinocytes, and other cell types (1-3).