Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Capillary Electrophoresis: Applications

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

Ion-Exchange Chromatography

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...

Electrophoresis: Overview

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...

Mass Spectrometry: Complex Analysis

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Large language models (LLMs) in radiography research: A narrative review.

Radiography (London, England : 1995)·2025

Same author

The human vermiform appendix: morphological and anatomical review.

Problemy sotsial'noi gigieny, zdravookhraneniia i istorii meditsiny·2023

Same author

PIN diode driver for NMR and MRI.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2019

Same author

High-frequency ultrasound measurement of dermis and subcutaneous fat in the newborn infant.

Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)·2016

Same author

The impact of routine histopathological examination on cholecystectomy specimens from an Asian demographic.

Annals of the Royal College of Surgeons of England·2012

Same author

Quality improvement programme for diabetes care in family practice settings in Dubai.

Eastern Mediterranean health journal = La revue de sante de la Mediterranee orientale = al-Majallah al-sihhiyah li-sharq al-mutawassit·2007

Search research articles

Related Experiment Video

Updated: Jun 3, 2026

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Published on: March 8, 2020

Steroid analysis by micellar electrokinetic capillary chromatography.

A A Mohammad¹, J R Petersen, M G Bissell

¹Departments of Pathology and Clinical Chemistry, University of Texas Medical Branch, Galveston, TX.

Methods in Molecular Medicine

|March 5, 2011

Summary

Measuring a profile of steroids offers a more comprehensive understanding of steroid imbalances than single measurements. This approach aids in diagnosing conditions like hirsutism and acne by revealing complex hormonal patterns.

More Related Videos

Free Radicals in Chemical Biology: from Chemical Behavior to Biomarker Development

Free Radicals in Chemical Biology: from Chemical Behavior to Biomarker Development

Published on: April 15, 2013

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples

Published on: October 1, 2016

Related Experiment Videos

Last Updated: Jun 3, 2026

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Published on: March 8, 2020

Free Radicals in Chemical Biology: from Chemical Behavior to Biomarker Development

Free Radicals in Chemical Biology: from Chemical Behavior to Biomarker Development

Published on: April 15, 2013

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples

Published on: October 1, 2016

Area of Science:

Endocrinology
Biochemistry
Clinical Medicine

Background:

Steroids are crucial for specific physiological functions, including sexual differentiation, reproduction, and metabolic regulation.
Imbalances in steroid secretion are linked to numerous clinical disorders such as Addison's disease, Cushing's syndrome, and hypertension.
Understanding steroid pathophysiology often requires evaluating multiple steroid levels due to their cumulative effects.

Purpose of the Study:

To highlight the clinical significance of steroids in regulating specific physiological processes.
To underscore the importance of assessing steroid profiles for a comprehensive understanding of endocrine disorders.
To demonstrate the utility of steroid profiling in clinical diagnostics.

Main Methods:

Review of existing clinical and biochemical studies on steroid function and imbalance.
Analysis of the diagnostic benefits of measuring multiple steroids simultaneously.
Case study example illustrating steroid profiling for conditions like hirsutism.

Main Results:

Steroid imbalances, resulting from under- or oversecretion, are implicated in a wide range of diseases.
Measuring a profile of steroids provides greater insight into disease pathophysiology than single steroid measurements.
Steroid profiling has shown promise in understanding complex conditions such as hirsutism and acne.

Conclusions:

Steroid profiling is a valuable diagnostic tool for complex endocrine and metabolic disorders.
Comprehensive steroid analysis enhances the understanding of diseases caused by hormonal imbalances.
Future research should continue to leverage steroid profiling for improved clinical diagnostics and patient management.