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Updated: Jun 3, 2026

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method
Published on: December 25, 2016
Plasma nitrite and nitrate determination
T Ueda1, T Maekawa, K Nakamura
1Department of Anesthesiology-Resuscitology, Yamaguchi School of Medicine, Ube, Japan.
Abstract:
The concentrations of nitrite and nitrate in blood plasma are important in clinical situations, because they are now known to be the spontaneous chemical products of nitric oxide (NO) in blood (1). NO is a mediator with numerous functions, including the regulation of vascular tone (2) and cell-to-cell communication (3). It is also thought to be one of the chief pathogenic factors causing shock and organ dysfunction seen in critically ill patients (4). Because NO is rapidly oxidized to nitrite and nitrate in blood, these are used as the markers of NO generation.
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