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Related Concept Videos

2° Amines to N-Nitrosamines: Reaction with NaNO201:20

2° Amines to N-Nitrosamines: Reaction with NaNO2

Secondary amines react with nitrous acid to form N-nitrosamines, as depicted in Figure 1. Nitrous acid, a weak and unstable acid, is formed in situ from an aqueous solution of sodium nitrite and strong acids, such as hydrochloric acid or sulfuric acid, in cold conditions. In the presence of an acid, the nitrous acid gets protonated. The subsequent loss of water results in the formation of the electrophile known as nitrosonium ion.
Inorganic Nitrogen Assimilation01:22

Inorganic Nitrogen Assimilation

Nitrogen is an essential element in biological systems, forming a crucial component of proteins, nucleic acids, and other cellular constituents. Many bacteria and archaea acquire nitrogen in the form of nitrate (NO₃⁻) or ammonia (NH₃), which are then assimilated into biomolecules through specific enzymatic pathways.Assimilatory Nitrate ReductionWhen nitrate enters the cell, it undergoes a two-step reduction process known as assimilatory nitrate reduction. Initially, the enzyme nitrate reductase...
Precipitation Titration: Overview01:26

Precipitation Titration: Overview

Precipitation titration involves the reaction of a titrant and an analyte to generate an insoluble precipitate. While precipitation titration uses various precipitating agents, silver nitrate is the most common precipitating reagent; titrations involving Ag+ are called argentometric titrations. Usually, the endpoint in a precipitation titration can be detected by visual indicators.
A precipitation titration curve demonstrates the change in concentration of the titrant or analyte upon adding the...

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Related Experiment Video

Updated: Jun 3, 2026

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method
08:25

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method

Published on: December 25, 2016

Plasma nitrite and nitrate determination.

T Ueda1, T Maekawa, K Nakamura

  • 1Department of Anesthesiology-Resuscitology, Yamaguchi School of Medicine, Ube, Japan.

Methods in Molecular Medicine
|March 5, 2011
PubMed
Summary
This summary is machine-generated.

Nitrite and nitrate in blood plasma are key indicators of nitric oxide (NO) generation. Measuring these stable NO breakdown products helps assess NO

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Platelet-based Detection of Nitric Oxide in Blood by Measuring VASP Phosphorylation
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Last Updated: Jun 3, 2026

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method
08:25

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method

Published on: December 25, 2016

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
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Platelet-based Detection of Nitric Oxide in Blood by Measuring VASP Phosphorylation
07:13

Platelet-based Detection of Nitric Oxide in Blood by Measuring VASP Phosphorylation

Published on: January 7, 2019

Area of Science:

  • Biochemistry
  • Clinical Chemistry

Background:

  • Nitrite and nitrate concentrations in blood plasma are crucial clinical markers.
  • These compounds are the stable end products of nitric oxide (NO) in vivo.
  • Nitric oxide (NO) is a vital mediator involved in vascular tone regulation and cell communication.

Purpose of the Study:

  • To highlight the clinical significance of plasma nitrite and nitrate levels.
  • To establish nitrite and nitrate as reliable markers for nitric oxide (NO) generation in blood.

Main Methods:

  • Measurement of nitrite and nitrate concentrations in blood plasma.
  • Utilizing established biochemical assays for accurate quantification.

Main Results:

  • Plasma nitrite and nitrate levels directly correlate with nitric oxide (NO) production.
  • These metabolites serve as dependable indicators of NO activity in the bloodstream.

Conclusions:

  • Plasma nitrite and nitrate are essential biomarkers for assessing nitric oxide (NO) status.
  • Their measurement provides valuable insights into physiological and pathological processes involving NO.