Related Experiment Video
Updated: Jun 3, 2026
09:42
Development of an Economical DNA Delivery System by "Acufection" and its Application to Skin Research
Published on: April 19, 2017
Preparations for particle-mediated gene transfer using the accell® gene gun
M D Macklin1, R J Drape, W F Swain
1PowderJect Vaccines Inc., Madison, WI.
Methods in Molecular Medicine
|March 5, 2011
Summary
Particle-mediated delivery uses accelerated microparticles to deliver various molecules into cells. This versatile technique enables genetic engineering in plants and material transfer across diverse biological systems.
Area of Science:
- Biotechnology
- Cell Biology
- Molecular Biology
Background:
- Particle-mediated delivery is a method for intracellular material transfer.
- It involves coating substances onto microparticles and accelerating them to penetrate cells.
Purpose of the Study:
- To introduce foreign substances like DNA into cells.
- To develop genetically engineered plants.
- To explore the broad applicability of particle-mediated delivery.
Main Methods:
- Coating dense sub-cellular sized particles (0.5-5 mm) with materials.
- Accelerating coated particles to high velocities for cell penetration.
- Application across various cell and tissue types in vivo, ex vivo, and in vitro.
Main Results:
- Successful transfer of DNA into plant cells, leading to genetic engineering.
- Demonstrated general applicability for delivering DNA, RNA, proteins, peptides, and pharmacological compounds.
- Proven effectiveness in a wide range of biological systems.
Conclusions:
- Particle-mediated delivery is a robust and versatile method for intracellular delivery.
- The technique has significant applications in plant biotechnology and beyond.
- It facilitates the transfer of diverse molecules into various cell types.
