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Related Experiment Video

Updated: Jun 3, 2026

Correlative Light Electron Microscopy (CLEM) for Tracking and Imaging Viral Protein Associated Structures in Cryo-immobilized Cells

Correlative Light Electron Microscopy (CLEM) for Tracking and Imaging Viral Protein Associated Structures in Cryo-immobilized Cells

Published on: September 7, 2018

lmmunoelectron Microscopic Characterization of HCV.

S Watanabe¹, M Kaito, M Kohara

¹Health Administration Center, Mie University, Mie, Japan.

Methods in Molecular Medicine

|March 5, 2011

Summary

Electron microscopy visualizes viruses using negative staining. Immunoelectron microscopy enhances sensitivity by concentrating viral particles with antibodies, improving detection in clinical samples.

Area of Science:

Virology
Microscopy
Immunology

Background:

Electron microscopy (EM) enabled viral particle visualization in 1939.
Negative staining EM provides high-resolution images by contrasting viruses against heavy metal atoms.
A major limitation of negative staining EM is the need for high viral concentrations, often exceeding those in clinical samples.

Purpose of the Study:

To address the low sensitivity of standard electron microscopy for detecting viruses in clinical specimens.
To introduce and evaluate immunoelectron microscopy (IEM) as a method to enhance viral particle detection.

Main Methods:

Utilizing negative staining electron microscopy.
Employing specific antibodies to form immune complexes with viral particles, thereby concentrating them.

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Comparing the sensitivity of IEM with standard EM for viral detection.

Main Results:

Standard EM requires very high viral concentrations (10^9-10^12 particles/mL).
Immunoelectron microscopy enhances viral particle concentration through antigen-antibody complex formation.
IEM improves the sensitivity of electron microscopy by approximately 1000-fold.

Conclusions:

Immunoelectron microscopy significantly increases the sensitivity of viral detection compared to standard electron microscopy.
This enhanced sensitivity facilitates the identification of viruses in clinical specimens with low viral loads.
IEM offers the dual advantage of improved detection and identification of viral immunologic specificity.