Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Pulmonary Tuberculosis IV

Pulmonary Tuberculosis IV

Tuberculosis, more commonly referred to as TB, is an infectious disease stemming from Mycobacterium tuberculosis. While it primarily impacts the lungs, TB can also affect other body areas. Given its severity and global impact, timely and accurate diagnosis is crucial for controlling its spread and improving patient outcomes.
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Optimization of a real-time RT-PCR assay reveals an increase of genogroup I norovirus in the clinical setting.

Journal of virological methods·2011

Same author

Quantification of M. tuberculosis DNA in Sputum During the Treatment of Pulmonary Tuberculosis.

Methods in molecular medicine·2011

Same author

Microaerophilic induction of the alpha-crystallin chaperone protein homologue (hspX) mRNA of Mycobacterium tuberculosis.

Journal of bacteriology·2001

Same author

An increase in expression of a Mycobacterium tuberculosis mycolyl transferase gene (fbpB) occurs early after infection of human monocytes.

Molecular microbiology·2001

Same author

Identifying Mycobacterium tuberculosis virulence determinants - new technologies for a difficult problem.

Trends in microbiology·2000

Same author

Measurement of sputum Mycobacterium tuberculosis messenger RNA as a surrogate for response to chemotherapy.

American journal of respiratory and critical care medicine·1999

Search research articles

Related Experiment Video

Updated: Jun 3, 2026

A Tuberculosis Molecular Bacterial Load Assay (TB-MBLA)

A Tuberculosis Molecular Bacterial Load Assay (TB-MBLA)

Published on: April 30, 2020

Isolation of M. tuberculosis RNA from Sputum.

L E Desjardin¹

¹VAMC Research Service, Iowa City, IA.

Methods in Molecular Medicine

|March 5, 2011

Summary

Analyzing Mycobacterium tuberculosis (MTB) mRNA offers insights into pathogenesis and drug susceptibility. RNA analysis can also evaluate treatment efficacy in tuberculosis patients.

Area of Science:

Microbiology
Molecular Biology
Clinical Research

Background:

RNA analysis is crucial in basic research and clinical applications.
mRNA transcripts in Mycobacterium tuberculosis (MTB) provide insights into pathogenesis.
MTB mRNA levels correlate with bacterial viability in drug-treated cultures, offering an alternative to traditional assays.

Purpose of the Study:

To highlight the importance of MTB mRNA analysis in understanding pathogenesis.
To present MTB mRNA analysis as a viable alternative for assessing drug susceptibility.
To emphasize the application of MTB RNA isolation in evaluating tuberculosis treatment efficacy.

Main Methods:

Analysis of mRNA transcripts in Mycobacterium tuberculosis (MTB) bacilli.

More Related Videos

Matrix-based DNA Extraction for Targeted Next-Generation Sequencing on Decontaminated Sputum Samples

Matrix-based DNA Extraction for Targeted Next-Generation Sequencing on Decontaminated Sputum Samples

Published on: June 6, 2025

A Novel Microdissection Approach to Recovering Mycobacterium tuberculosis Specific Transcripts from Formalin Fixed Paraffin Embedded Lung Granulomas

A Novel Microdissection Approach to Recovering Mycobacterium tuberculosis Specific Transcripts from Formalin Fixed Paraffin Embedded Lung Granulomas

Published on: June 5, 2014

Related Experiment Videos

Last Updated: Jun 3, 2026

A Tuberculosis Molecular Bacterial Load Assay (TB-MBLA)

A Tuberculosis Molecular Bacterial Load Assay (TB-MBLA)

Published on: April 30, 2020

Matrix-based DNA Extraction for Targeted Next-Generation Sequencing on Decontaminated Sputum Samples

Matrix-based DNA Extraction for Targeted Next-Generation Sequencing on Decontaminated Sputum Samples

Published on: June 6, 2025

A Novel Microdissection Approach to Recovering Mycobacterium tuberculosis Specific Transcripts from Formalin Fixed Paraffin Embedded Lung Granulomas

A Novel Microdissection Approach to Recovering Mycobacterium tuberculosis Specific Transcripts from Formalin Fixed Paraffin Embedded Lung Granulomas

Published on: June 5, 2014

Correlation of MTB mRNA levels with bacterial viability in drug-treated cultures.

Isolation of MTB RNA for evaluating treatment efficacy in patients.

Main Results:

mRNA expression patterns in MTB can elucidate models of pathogenesis.
MTB mRNA levels serve as an indicator of bacterial viability.
RNA isolation from MTB is applicable for assessing treatment response in tuberculosis patients.

Conclusions:

MTB mRNA analysis is a valuable tool for both research and clinical practice.
RNA-based methods offer a promising alternative for drug susceptibility testing.
The isolation and analysis of MTB RNA are critical for monitoring treatment efficacy in tuberculosis patients.