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Updated: Jun 3, 2026

Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders
Published on: April 14, 2015
Structure elucidation of gluten-derived peptides by tandem mass spectrometry
P A van Veelen1, J W Drijfhout, F Koning
1Department of Immunohaematology and Bloodbank, University of Leiden, Leiden, The Netherlands.
Abstract:
In recent years there have been major developments in the field of mass spectrometry (MS) that permit the analysis and characterization of peptides and proteins at the femtomolar level (1-8). This chapter deals with the use of MS for the elucidation of peptide sequences of gliadin- and/or glutenin-derived fragments. It is meant to give an overview of what modern mass spectrometric methods can mean for your research in the biomolecular field rather than an in-depth step-by-step practical protocol. Important aspects are covered to give the reader an idea of what is needed to perform, and what can be expected from, this mass spectrometric application.
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