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Antigens Involved in Adaptive Immunity

Antigens Involved in Adaptive Immunity

An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
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Related Experiment Video

Updated: Jun 3, 2026

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis

Published on: October 15, 2021

Characterization of HLA-DQ-Specific Peptide-Binding Motifs.

Y van de Wal¹, R Amons, F Koning

¹Department of Immunohaematology and Bloodbank, University of Leiden, Leiden, The Netherlands.

Methods in Molecular Medicine

|March 5, 2011

Summary

Understanding Human Leukocyte Antigen (HLA) allele associations with immune disorders is key. Characterizing HLA peptide-binding motifs helps identify disease-causing peptides and antigens, advancing immunology research.

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Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis

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Area of Science:

Immunology
Molecular Biology
Genetics

Background:

Immune disorders are linked to specific Human Leukocyte Antigen (HLA) alleles.
The exact mechanisms of these HLA-disease associations are not fully understood.
Peptides bound to HLA molecules are implicated in disease pathogenesis.

Purpose of the Study:

To characterize peptide-binding motifs of disease-associated HLA class II molecules.
To identify potential disease-inducing peptides and antigens by understanding HLA-peptide interactions.

Main Methods:

Analyzing naturally processed HLA-bound peptides.
Utilizing peptide display libraries (M13 bacteriophage and synthetic).
Assessing peptide-binding requirements via peptide analogs with single amino acid substitutions.

Main Results:

Defined allele-dependent peptide-binding motifs based on anchor residue preferences.
Demonstrated the utility of these motifs for predicting peptide binding to specific HLA molecules.

Conclusions:

Characterizing HLA peptide-binding motifs is crucial for identifying disease-associated peptides.
This approach aids in understanding the molecular basis of HLA-disease associations.
Predictive motifs offer a valuable tool for immunological research and disease investigation.