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Related Concept Videos

In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...

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Related Experiment Video

Updated: Jun 3, 2026

In Situ Hybridization for the Precise Localization of Transcripts in Plants
12:15

In Situ Hybridization for the Precise Localization of Transcripts in Plants

Published on: November 23, 2011

In situ hybridization.

C G Beckett1, P J Ciclitira

  • 1Department of Gastroenterology, Bradford Royal Informary, Bradford, West Yorkshire, UK.

Methods in Molecular Medicine
|March 5, 2011
PubMed
Summary
This summary is machine-generated.

In situ hybridization allows precise gene identification and expression analysis within tissue context. This technique relies on hybridizing labeled nucleic acid probes to complementary mRNA or DNA sequences.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • In situ hybridization is a powerful technique for visualizing nucleic acid sequences within their native cellular or tissue environment.
  • Developed in 1960, it allows for the spatial localization of specific DNA or RNA molecules.

Purpose of the Study:

  • To describe the fundamental principles and applications of in situ hybridization.
  • To highlight its utility in identifying genes and analyzing gene expression patterns.

Main Methods:

  • Utilizes the hybridization of a labeled nucleic acid probe.
  • The probe binds to complementary sequences of messenger RNA (mRNA) or DNA within the tissue sample.
  • Maintains the topographical context of the target sequence.

Main Results:

  • Enables specific identification of genes within cells and tissues.
  • Allows for the direct visualization of gene expression by detecting mRNA levels.
  • Preserves the spatial organization of the biological sample.

Conclusions:

  • In situ hybridization is a crucial method for understanding gene function and regulation in a spatial context.
  • The technique's ability to identify genes and gene expression without disrupting tissue architecture is invaluable.
  • Its continued application is essential in various fields of biological research.