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Updated: Jun 3, 2026
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In Situ Hybridization for the Precise Localization of Transcripts in Plants
Published on: November 23, 2011
In situ hybridization
1Department of Gastroenterology, Bradford Royal Informary, Bradford, West Yorkshire, UK.
Methods in Molecular Medicine
|March 5, 2011
Abstract:
In situhybridization permits specific identification of genes and gene expression without removing the target sequence from its topographical surroundings. The technique was described simultaneously in 1960 by two groups (1,2). It depends on the hybridization of a labeled nucleic acid probe to a complementary sequence of tissue mRNA or DNA.
Related Concept Videos
02:31In-situ Hybridization
In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
02:07FISH - Fluorescent In-situ Hybridization
Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...