Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Conjugated Proteins

Conjugated Proteins

Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Movement and habitat selection of a large carnivore in response to human infrastructure differs by life stage.

Movement ecology·2022

Same author

Social environment shapes female settlement decisions in a solitary carnivore.

Behavioral ecology : official journal of the International Society for Behavioral Ecology·2022

Same author

Breast cancer in systemic lupus.

Lupus·2016

Same author

Instrument for precision long-term β-decay rate measurements.

The Review of scientific instruments·2015

Same author

Breast cancer in systemic lupus erythematosus.

Oncology·2013

Same author

Assessment of bronchoreversibility in severe emphysema.

The European respiratory journal·2010

Search research articles

Related Experiment Video

Updated: Jun 3, 2026

Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay

Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay

Published on: September 14, 2014

Characterization of HIV gp120 Envelope Glycoprotein by Lectin Analysis.

G J Gram¹, J E Hansen

¹Department of Infectious Diseases, University of Copenhagen, Hvidovere Hospital, Copenhagen, Denmark.

Methods in Molecular Medicine

|March 5, 2011

Summary

The human immunodeficiency virus (HIV) envelope glycoprotein gp120 has numerous N-glycosylation sites, making up half its mass. This glycosylation is crucial for viral infection by facilitating binding to CD4 molecules.

More Related Videos

Detection of Neutralization-sensitive Epitopes in Antigens Displayed on Virus-Like Particle (VLP)-Based Vaccines Using a Capture Assay

Detection of Neutralization-sensitive Epitopes in Antigens Displayed on Virus-Like Particle (VLP)-Based Vaccines Using a Capture Assay

Published on: February 10, 2022

Development of Cell-type specific anti-HIV gp120 aptamers for siRNA delivery

Development of Cell-type specific anti-HIV gp120 aptamers for siRNA delivery

Published on: June 23, 2011

Related Experiment Videos

Last Updated: Jun 3, 2026

Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay

Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay

Published on: September 14, 2014

Detection of Neutralization-sensitive Epitopes in Antigens Displayed on Virus-Like Particle (VLP)-Based Vaccines Using a Capture Assay

Detection of Neutralization-sensitive Epitopes in Antigens Displayed on Virus-Like Particle (VLP)-Based Vaccines Using a Capture Assay

Published on: February 10, 2022

Development of Cell-type specific anti-HIV gp120 aptamers for siRNA delivery

Development of Cell-type specific anti-HIV gp120 aptamers for siRNA delivery

Published on: June 23, 2011

Area of Science:

Virology
Glycobiology
Immunology

Background:

The human immunodeficiency virus (HIV) causes AIDS.
HIV's envelope glycoprotein, gp120, is heavily glycosylated.
N-glycosylation sites are critical for viral structure and function.

Purpose of the Study:

To investigate the role of N-glycosylation in HIV gp120.
To understand the contribution of glycosylation to gp120's molecular mass.
To explore the significance of gp120 glycosylation in viral entry.

Main Methods:

Analysis of gp120 amino acid sequence for N-glycosylation sites (Asn-X-Ser/Thr, X≠Pro).
Quantification of glycosylation contribution to gp120 molecular mass.
Review of literature on gp120-CD4 binding and viral fusion.

Main Results:

Approximately 24 potential N-glycosylation sites were identified in gp120.
All identified sites are utilized, contributing to about 50% of gp120's molecular mass.
gp120 binding to CD4 is an essential early step in HIV infection.

Conclusions:

HIV gp120 is extensively N-glycosylated, impacting its structure and function.
Glycosylation plays a significant role in the initial stages of HIV infection, including CD4 binding and cell fusion.