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Amyloid Fibrils03:03

Amyloid Fibrils

Amyloid fibrils are aggregates of misfolded proteins.  Under most circumstances, misfolded proteins are either refolded by chaperone proteins or degraded by the proteasome. However, in the case of a mutation or a disease, these proteins can accumulate to form large clusters and often further assemble to form elongated fibers, called fibrils. 
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Isolation of Soluble and Insoluble PrP Oligomers in the Normal Human Brain
11:29

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Published on: October 3, 2012

Prion protein as copper-binding protein at the synapse.

H A Kretzschmar1, T Tings, A Madlung

  • 1Institute for Neuropathology, Gottingen, Germany.

Methods in Molecular Medicine
|March 5, 2011
PubMed
Summary
This summary is machine-generated.

Researchers investigated the function of prion proteins (PrPC), exploring potential binding partners. Biochemical analyses suggest PrPC may regulate cholinergic receptors at the neuromuscular junction.

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Area of Science:

  • Neuroscience
  • Biochemistry
  • Cell Biology

Background:

  • Prion proteins (PrPC) are cell surface proteins with incompletely understood functions.
  • Various potential binding partners for PrPC have been identified using biochemical methods.
  • The biological significance of these PrPC interactions remains largely unproven.

Purpose of the Study:

  • To explore the function of prion proteins (PrPC).
  • To identify and characterize binding partners of PrPC.
  • To investigate the potential role of PrPC in regulating cholinergic receptors.

Main Methods:

  • Application of biochemical methods to identify PrPC binding partners.
  • Analysis of cell surface PrPC interactions.
  • Biochemical analyses of chicken PrPC.

Main Results:

  • Several potential binding partners for PrPC were identified, including glial fibrillary acidic protein, Bcl-2, molecular chaperones, amyloid precursor-like protein 1, and the 37-kDa laminin receptor.
  • The biological significance of these identified binding partners for PrPC function has not been demonstrated.
  • Biochemical analyses of chicken PrPC led to a hypothesis regarding its role in receptor regulation.

Conclusions:

  • Despite identifying several potential binding partners, the biological significance of PrPC interactions is not yet established.
  • PrPC may play a regulatory role in the expression of cholinergic receptors at the neuromuscular endplate, as suggested by studies on chicken PrPC.