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Related Experiment Video

Updated: Jun 3, 2026

Control of Cell Adhesion using Hydrogel Patterning Techniques for Applications in Traction Force Microscopy
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Control of Cell Adhesion using Hydrogel Patterning Techniques for Applications in Traction Force Microscopy

Published on: January 29, 2022

Controlling fibroblast adhesion with pH modified polyelectrolyte multilayers.

Marcus S Niepel1, Dieter Peschel, Thomas Groth

  • 1Biomedical Materials Group, Department of Pharmaceutical Technology and Biopharmacy, Institute of Pharmacy, Martin Luther University Halle-Wittenberg, Halle, Saale, Germany.

The International Journal of Artificial Organs
|March 5, 2011
PubMed
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Surface coatings made from polyethyleneimine (PEI) and heparin (HEP) can be tuned using pH to control cell adhesion. This biomaterial surface modification is crucial for implant integration and biomedical applications.

Area of Science:

  • Biomaterials Science
  • Surface Chemistry
  • Cell Biology

Background:

  • Cell adhesion to biomaterials is vital for implant integration and tissue regeneration.
  • Surface properties significantly influence cell behavior, including adhesion, growth, and differentiation.
  • Controlling surface characteristics is key for developing advanced biomedical applications.

Purpose of the Study:

  • To design self-assembled polyelectrolyte multilayer (PEM) coatings using polyethyleneimine (PEI) and heparin (HEP) on glass surfaces.
  • To investigate how pH-controlled surface modification affects primary human dermal fibroblast adhesion and proliferation.
  • To explore the potential of tailored PEM coatings for biomedical applications requiring specific cell interactions.

Main Methods:

  • Utilized the layer-by-layer (LbL) technique to create PEI/HEP PEM coatings on glass substrates.

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Composite Scaffolds of Interfacial Polyelectrolyte Fibers for Temporally Controlled Release of Biomolecules
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Published on: August 19, 2015

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Last Updated: Jun 3, 2026

Control of Cell Adhesion using Hydrogel Patterning Techniques for Applications in Traction Force Microscopy
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Control of Cell Adhesion using Hydrogel Patterning Techniques for Applications in Traction Force Microscopy

Published on: January 29, 2022

Composite Scaffolds of Interfacial Polyelectrolyte Fibers for Temporally Controlled Release of Biomolecules
11:13

Composite Scaffolds of Interfacial Polyelectrolyte Fibers for Temporally Controlled Release of Biomolecules

Published on: August 19, 2015

  • Manipulated the pH of the heparin solution during the terminal layer deposition to alter surface properties and wettability.
  • Assessed cell adhesion, morphology, and proliferation of human dermal fibroblasts on the modified surfaces, with and without fibronectin pre-adsorption.

    • Main Results:

    • Surface wettability and multilayer growth were controllable by adjusting the pH of the terminal heparin layers.
    • Plain terminal layers exhibited cytophobic (cell-repelling) properties.
    • Fibronectin pre-adsorption significantly influenced cell adhesion and morphology in a pH-dependent manner.
    • PEM coatings that impaired cell adhesion also inhibited fibroblast proliferation, while those promoting adhesion enhanced growth.

    Conclusions:

    • Heparin-terminated PEM coatings are suitable for applications needing cell-repellent surfaces.
    • Polyethyleneimine-terminated PEM coatings can be utilized to promote cell adhesion and growth on implant surfaces.
    • pH-controlled surface modification of PEMs offers a versatile strategy for tailoring biomaterial interactions with cells.