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Updated: Jun 3, 2026

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

Published on: October 1, 2014

Three-dimensional automated nanoparticle tracking using Mie scattering in an optical microscope.

J-M Gineste¹, P Macko, E A Patterson

¹Systems Toxicology Unit, Institute for Health and Consumer Protection, European Commission DG Joint Research Centre, Italy.

Journal of Microscopy

|March 8, 2011

Summary

This summary is machine-generated.

This study presents a novel optical microscopy method for precisely tracking nanoparticles in 3D. The technique enables automated, quantitative identification and localization of nanoparticles with high temporal resolution.

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A Protocol for Real-time 3D Single Particle Tracking

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

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Last Updated: Jun 3, 2026

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

Published on: October 1, 2014

A Protocol for Real-time 3D Single Particle Tracking

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

Area of Science:

Optical Microscopy
Nanotechnology
Biophysics

Background:

Accurate nanoparticle tracking is crucial for various scientific and industrial applications.
Conventional methods can be complex or lack the required resolution.
Developing simple yet effective optical techniques for nanoparticle analysis is an ongoing challenge.

Purpose of the Study:

To develop an automated method for three-dimensional (3D) nanoparticle identification and tracking.
To achieve high temporal resolution for dynamic studies of nanoparticles.
To leverage forward light scattering in a standard optical microscope for quantitative analysis.

Main Methods:

Utilized forward light scattering from nanoparticles (10-50nm diameter) in an inverted optical microscope.
Employed objective lens oscillation via a piezo actuator and a minimized condenser aperture.
Implemented automated data processing in both time and spatial domains for particle localization.

Main Results:

Achieved automatic and quantitative 3D localization and tracking of nanoparticles.
Demonstrated a temporal resolution of 200ms for particle tracking.
Quantified localization precision: ~50nm along the light path and ~5nm perpendicular to it for 50nm nanoparticles.

Conclusions:

The developed optical microscopy technique offers a simple, automated, and quantitative solution for 3D nanoparticle tracking.
The method's high temporal resolution and precision make it suitable for dynamic nanoscale investigations.
Potential applications span nanoparticle processing, nanobiotechnology, pharmaceuticals, and food processing.