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Structural and functional analysis of the N-terminal region of death receptor 5
Zhang Feng1, Chen Cai-feng, Liu Shi-lian
1National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences,CAMS and PUMC, Beijing 100005, China.
Objective:
To investigate the structure and function of the N-terminal region (NTR) of death receptor 5 (DR5).
Methods:
A series of deletions of the DR5 extracellular domain (DR5-ECD) proteins were expressed in E.coli. and purified by affinity chromatography. The binding ability of these deletant proteins to AD5-10, a mouse anti-human DR5 monoclonal antibody, was evaluated by immunoblotting and ELISA.
Results:
Recombinant DR5-ECD proteins containing the NTR were recognized and bound by AD5-10, while the other deletant proteins without the NTR failed to interact with AD5-10.
Conclusion:
There is an AD5-10 targeting site in the NTR of DR5, which may play a role in developing novel immunotherapies for cancers.
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