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Microparticles in newborn cord blood: slight elevation after normal delivery
Sabrina Schweintzger1, Axel Schlagenhauf, Bettina Leschnik
1Department of Pediatrics and Adolescent Medicine, Medical University of Graz, Austria.
Thrombosis Research
|March 8, 2011
Summary
Newborns have more active microparticles than adults, impacting blood clotting. While microparticle concentration is similar, their procoagulant activity is higher in cord plasma, suggesting increased hemostatic system impact.
Area of Science:
- Hematology
- Neonatal Physiology
- Hemostasis
Background:
- Microparticles (MPs) play a dual role in hemostasis, potentially contributing to both normal function and hypercoagulability in newborns.
- Delivery processes may influence MP formation and activity.
Purpose of the Study:
- To compare microparticle concentration, procoagulant activity, and thrombin generation effects between newborn cord plasma and adult plasma.
- To elucidate the specific impact of microparticles on neonatal hemostasis.
Main Methods:
- Flow cytometry was used for microparticle enumeration and characterization.
- Enzyme-linked immunosorbent assay (ELISA) measured procoagulant activity via the prothrombinase reaction.
- Calibrated automated thrombography assessed thrombin generation in platelet-free plasma.
Main Results:
- Flow cytometry revealed comparable microparticle concentrations in newborn cord and adult plasma.
- ELISA demonstrated significantly higher procoagulant activity of microparticles in newborn cord plasma compared to adult plasma.
- Thrombin generation assays indicated increased microparticle activity in newborn plasma.
Conclusions:
- Newborn cord plasma exhibits higher microparticle procoagulant activity, not concentration, compared to adult plasma.
- Findings suggest a greater impact of microparticles on the neonatal hemostatic system.
- Comparable microparticle numbers do not support significant platelet activation during birth.
