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Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns

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Liquid-phase and dispersive liquid-liquid microextraction techniques with derivatization: recent applications in

Abdulmumin A Nuhu¹, Chanbasha Basheer, Bahruddin Saad

¹Department of Chemistry, King Fahd University of Petroleum and Minerals, KFUPM Box 1059, Dhahran 31261, Saudi Arabia.

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences

|March 8, 2011

Summary

Liquid phase microextraction (LPME) and dispersive liquid-liquid microextraction (DLLME), when combined with derivatization, offer efficient single-step analysis of biological samples. These methods enhance analyte enrichment for diverse applications, including trace metal determination.

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Published on: June 1, 2017

Area of Science:

Analytical Chemistry
Biochemistry
Environmental Science

Background:

Liquid phase microextraction (LPME) and dispersive liquid-liquid microextraction (DLLME) are powerful sample preparation techniques.
These methods provide high analyte enrichment in a single step, simplifying complex analyses.
Coupling microextraction techniques with derivatization significantly boosts their analytical utility, especially for biological samples.

Purpose of the Study:

To review recent advancements in combined LPME (including HF-LPME and SDME) and DLLME techniques.
To highlight their application in the analysis of diverse biological matrices.
To discuss the determination of organic compounds and trace metal ions using these methods.

Main Methods:

Focus on hollow fiber liquid-phase microextraction (HF-LPME), single drop microextraction (SDME), and dispersive liquid-liquid microextraction (DLLME).
Integration of various derivatization strategies tailored for specific analytes.
Application across multiple biological sample types: urine, blood, plasma, and hair.

Main Results:

Demonstrated high enrichment factors for target analytes in biological samples.
Successful application for analyzing polar and ionizable organic compounds.
Extended utility for the determination of trace metal ions like Hg, Pb, Co, and Se.

Conclusions:

Combined LPME and DLLME with derivatization represent a significant advancement in bioanalysis.
These techniques offer simplicity, efficiency, and broad applicability for complex sample matrices.
Future trends indicate continued development and expanded use in trace analysis.