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Related Concept Videos

The Cell Cycle Control System01:28

The Cell Cycle Control System

The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and function at the cell...
The Cell Cycle Control System02:11

The Cell Cycle Control System

The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
The Cell Cycle Control System02:11

The Cell Cycle Control System

The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
Positive Regulator Molecules02:39

Positive Regulator Molecules

Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
Positive Regulator Molecules01:45

Positive Regulator Molecules

To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
What is the Cell Cycle?00:56

What is the Cell Cycle?

The cell cycle refers to the sequence of events occurring throughout a typical cell’s life. In eukaryotic cells, the somatic cell cycle has two stages: the interphase and the mitotic phase. During interphase, the cell grows, performs its basic metabolic functions, copies its DNA, and prepares for mitotic cell division. Then, during mitosis and cytokinesis, the cell divides its nuclear and cytoplasmic materials, respectively. This generates two daughter cells that are identical to the original...

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Related Experiment Video

Updated: Jun 3, 2026

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
08:13

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

Published on: September 26, 2025

Cell cycle: who turns the crank?

John J Tyson1, Bela Novak

  • 1Department of Biological Sciences, Virginia Polytechnic Institute & State University, Blacksburg, VA 24060, USA. tyson@vt.edu

Current Biology : CB
|March 8, 2011
PubMed
Summary

A single CDK-cyclin fusion protein's oscillating activity drives yeast cell progression through DNA replication, mitosis, and cell division. This finding reveals a simplified mechanism for cell cycle control.

Area of Science:

  • Cell biology
  • Molecular biology
  • Biochemistry

Background:

  • Cell cycle progression is regulated by cyclin-dependent kinases (CDKs) and cyclins.
  • Understanding the minimal components required for cell cycle control is crucial.

Purpose of the Study:

  • To investigate if a single CDK-cyclin fusion protein can autonomously drive cell cycle progression.
  • To elucidate the role of oscillating activity in cell division.

Main Methods:

  • Construction and expression of a synthetic CDK-cyclin fusion protein in yeast.
  • Live-cell imaging to monitor cell cycle events.
  • Biochemical assays to confirm protein activity.

Main Results:

  • The engineered CDK-cyclin fusion protein exhibited robust oscillating activity.

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Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

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Last Updated: Jun 3, 2026

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
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Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

Published on: September 26, 2025

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
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Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

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  • This oscillation was sufficient to drive yeast cells through DNA replication, mitosis, and cytokinesis.
  • The fusion protein mimicked the essential functions of endogenous cell cycle regulators.

    • Conclusions:

    • A single, oscillating CDK-cyclin fusion protein can serve as a sole driver of cell cycle progression.
    • This demonstrates a simplified, yet functional, model for cell cycle control.
    • Highlights the importance of oscillatory dynamics in biological systems.