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Analyzing Spatial Learning and Prosocial Behavior in Mice Using the Barnes Maze and Damsel-in-Distress Paradigms
Published on: November 17, 2018
NMDA-complexes linked to spatial memory performance in the Barnes maze in CD1 mice
Maryam Ghafari1, Sudarshan S Patil, Harald Höger
1Department of Pediatrics, Medical University of Vienna, Währinger Gürtel 18, A-1090 Vienna, Austria.
Behavioural Brain Research
|March 8, 2011
Summary
The N-methyl-d-aspartic acid receptor (NMDAR) NR1 dimer complex levels increased during spatial memory formation in mice. This suggests NMDAR complexes, not just subunits, are crucial for memory processes.
Area of Science:
- Neuroscience
- Molecular Biology
- Cognitive Science
Background:
- The N-methyl-d-aspartic acid receptor (NMDAR) is vital for memory formation, including spatial memory.
- While NMDAR subunits are implicated, the role of intact NMDAR complexes in memory remains underexplored.
Purpose of the Study:
- To investigate the involvement of native NMDAR complexes in spatial memory formation.
- To compare NMDAR complex composition between trained and control mice.
Main Methods:
- CD1 mice were trained in the Barnes maze task.
- Hippocampal membrane proteins were extracted and analyzed using Blue Native PAGE followed by immunoblotting.
- NMDAR subunit composition within complexes was determined by 2D gel electrophoresis and immunoblotting.
Main Results:
- A specific NMDAR complex, likely an NR1 dimer (146-242 kDa), showed significantly altered levels in trained mice compared to controls.
- Multiple NMDAR complexes containing NR1, NR2A, and NR2B subunits were identified, primarily as heteropolymers.
- Increased NR1 dimer levels correlated with memory formation, suggesting a direct or indirect role.
Conclusions:
- The study highlights the importance of analyzing native NMDAR complexes rather than individual subunits for understanding memory mechanisms.
- Findings suggest that the NR1 dimer complex plays a significant role in spatial memory formation in CD1 mice.
- This research provides a molecular basis for future pharmacological and molecular investigations into NMDAR function in memory.
