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Updated: Jun 3, 2026
09:46
Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris
Published on: February 25, 2010
Generation and screening of Pichia pastoris strains with enhanced protein production by use of microengraving
Vasiliki Panagiotou1, Kerry Routenberg Love, Bo Jiang
1Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave., Building 76-253, Cambridge, MA 02139, USA.
Applied and Environmental Microbiology
|March 8, 2011
Abstract:
The selection of highly productive cell lines remains a key step for manufacturing therapeutic proteins. Microengraving was used to screen chemically mutagenized populations of Pichia pastoris for increased production of an Fc fragment. Clones retrieved following three rounds of mutagenesis yielded titers 2.65-fold greater than those of the parental strain.
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