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Related Experiment Video

Updated: Jun 3, 2026

Processing of Bronchoalveolar Lavage Fluid and Matched Blood for Alveolar Macrophage and CD4+ T-cell Immunophenotyping and HIV Reservoir Assessment
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Processing of Bronchoalveolar Lavage Fluid and Matched Blood for Alveolar Macrophage and CD4+ T-cell Immunophenotyping and HIV Reservoir Assessment

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Quantitative HIV Culture.

J R Lane1

  • 1SRA Technologies, Inc., Rockville, MD.

Methods in Molecular Medicine
|March 8, 2011
PubMed
Summary
This summary is machine-generated.

This study details a cell culture assay for isolating viruses, specifically detecting HIV in patient cells by measuring p24 gag protein. This method quantizes viral burden, reflecting cellular viral load.

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Last Updated: Jun 3, 2026

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Evaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy

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Area of Science:

  • Virology
  • Immunology
  • Cell Biology

Background:

  • Human Immunodeficiency Virus (HIV) isolation is crucial for diagnosis and research.
  • Quantifying viral burden in patients provides insights into disease progression.
  • Cell culture assays are standard for detecting viral presence and activity.

Purpose of the Study:

  • To describe a method for establishing cocultures for virus isolation using an endpoint dilution assay.
  • To present a cell culture assay for detecting HIV in peripheral blood mononuclear cells (PBMC).
  • To establish this assay as a measure of viral burden and cellular viral load.

Main Methods:

  • Utilizing a 96-well tissue culture plate for coculture.
  • Employing an endpoint dilution assay for virus isolation.
  • Detecting HIV by measuring the p24 gag protein in culture supernatant from patient PBMCs.

Main Results:

  • Successful establishment of cocultures for virus isolation.
  • Detection of HIV through p24 gag protein in culture supernatant.
  • Demonstration that the number of patient cells yielding a positive culture correlates with cellular viral load.

Conclusions:

  • The described procedure enables virus isolation via coculture in a 96-well plate.
  • The assay effectively detects HIV in patient PBMCs by monitoring p24 gag protein.
  • This method serves as a valuable tool for assessing viral burden and cellular viral load in research settings.