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Updated: Jun 3, 2026

Determining 3'-Termini and Sequences of Nascent Single-Stranded Viral DNA Molecules during HIV-1 Reverse Transcription in Infected Cells
Published on: January 30, 2019
Quantitation of HIV-1gag DNA and RNA From Single Frozen Cell Pellets
1Division of Retrovirology, Walter Reed Army Institute of Research, Rockville, MD.
Abstract:
The assessment of viral load in the blood, tissues, and bodily fluids of persons infected with human immunodeficiency virus is fundamental to defining the stage of disease (1-3), the effect of antiviral treatments to abate disease (4, 5), disease progression (6-8), and propensity for the transmission of disease (9,10) as well. Now accepted as a surrogate for all of these features of HIV-1 disease, specific guidelines have been adopted for the use of viral load measures in the clinical management of the disease (11).
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