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Updated: Jun 3, 2026

An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides
Published on: November 29, 2016
Mobility-Shift DNA-Binding Assay Using Gel Electrophoresis
1Department of Pharmacology and Therapeutics, College of Medicine, University of South Florida, Tampa, FL.
Abstract:
Transcription factors are induced in neurons to alter gene expression to adapt to brain injury caused by exposure to neurotoxins, neurodegeneration disease, or mechanical damage. These DNA-binding proteins bind to specific recognition sequences in the promoter region of genes to regulate transcription. Genes related to both regeneration and degeneration are regulated by transcription factors. Therefore, the fate of a neuron is determined by which transcription factors are expressed within a particular cell.
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