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Related Experiment Videos

gp33-38, an early human T cell activation antigen.

S Carrel1, S Salvi, P Isler

  • 1Ludwig Institute for Cancer Research, Lausanne Branch, Epalinges, Switzerland.

Journal of Immunology (Baltimore, Md. : 1950)
|March 15, 1990
PubMed
Summary

A novel T cell activation antigen, Me14/D12 (gp33-38), emerges early in T lymphocyte activation. Its expression precedes Interleukin-2 Receptor (IL-2R) gene transcripts, indicating a role in early T cell response.

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Area of Science:

  • Immunology
  • T cell biology
  • Molecular immunology

Background:

  • T cell activation involves complex molecular events and the expression of specific antigens.
  • Early activation markers are crucial for understanding T cell response dynamics.
  • The precise molecular players involved in early T cell activation require further elucidation.

Purpose of the Study:

  • To identify and characterize a novel activation antigen expressed early after T cell activation.
  • To investigate the temporal expression pattern of this antigen relative to other activation markers like IL-2R.
  • To explore the functional consequences of this antigen's expression and antibody binding.

Main Methods:

  • Characterization of the Me14/D12 antigen as a nondisulfide-linked heterodimer (gp33-38).

Related Experiment Videos

  • Analysis of Me14/D12 mRNA and surface protein expression kinetics following various T cell activation stimuli (PHA, Con A, PMA, anti-CD3 mAb).
  • Functional assays including IL-2 production and calcium (Ca2+) mobilization upon antibody binding to Me14/D12.

    • Main Results:

    • Me14/D12 antigen expression is induced by multiple activation stimuli and is absent in resting T cells.
    • Me14/D12 mRNA induction occurs significantly earlier (2h) than IL-2R mRNA (24h) in PHA-activated T cells.
    • Surface expression of Me14/D12 is detectable between 12-24h and maximal at 24-48h post-activation.
    • PMA and IFN-gamma can induce Me14/D12 expression on T leukemia cell lines.
    • Antibody binding to Me14/D12 on stimulated Jurkat cells triggers IL-2 production and Ca2+ mobilization.
    • Biochemical analysis confirms Me14/D12 is distinct from the CD69 molecular complex.

    Conclusions:

    • Me14/D12 (gp33-38) is a novel, early- T cell activation antigen.
    • Its expression kinetics suggest a role in the initial phases of T cell activation, preceding IL-2R expression.
    • Me14/D12 engagement can modulate T cell function, including IL-2 production and calcium signaling.