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Updated: Jun 3, 2026
Experimental Model of Ligature-Induced Peri-Implantitis in Mice
Published on: May 17, 2024
Immediate loading after implant placement following tooth extraction up-regulates cellular activity in the dog
Ryuta Sato1, Kenichi Matsuzaka, Eitoyo Kokubu
1Department of Clinical Pathophysiology, Tokyo Dental College, Chiba, Japan.
Objectives:
The aim of this study was to investigate cellular activity of the cervical portion of peri-implant tissue due to immediate loading after implant placement following tooth extraction from the dog mandible, in terms of morphological, immunohistochemical and molecular characteristics.
Material And Methods:
A sand-blasted implant was inserted into the root septum bone of each extraction socket and was connected to a superstructure made from resin and then covered with an expanded polytetrafluoroethylene membrane. Implants without the superstructure were used as the non-loading control group. Animals were sacrificed 1-3 weeks later and specimens were observed using light microscopy and mRNA levels were analyzed by real-time polymerase chain reaction.
Results:
The new bone formation ratio in the loading group at 3 weeks was significantly higher than in the non-loading group. Alkaline phosphatase (ALP)-positive cells were observed in tissues around the implant surface in both groups at each of the time periods. More osteocalcin (OCN)-positive cells were observed in the non-loading group than in the loading group at 2 weeks. The expression of ALP mRNA in the loading group was significantly up-regulated compared with the non-loading group (P<0.05). The expression of OCN mRNA in the loading group was significantly up-regulated compared with the non-loading group at 2 weeks (P<0.05).
Conclusion:
These results suggest that immediate loading after implant placement following tooth extraction osteogenic affects cellular activity of cervical portion of peri-implant tissue.
