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Related Concept Videos

Papillary Dermis01:11

Papillary Dermis

Dermis
The dermis might be considered the "core" of the integumentary system, as distinct from the epidermis and hypodermis. It contains blood and lymph vessels, nerves, and other structures, such as hair follicles and sweat glands. The dermis is made of two layers of connective tissue that comprise an interconnected mesh of elastin and collagenous fibers, produced by fibroblasts.
Papillary Layer
The papillary layer is made of loose, areolar connective tissue, which means the collagen and...

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Analysis of cell characterization using cell surface markers in the dermis.

Yuichi Hasebe1, Seiji Hasegawa, Noriko Hashimoto

  • 1Research Laboratories, Nippon Menard Cosmetic Co., Ltd., 2-7 Torimicho, Nishi-Ku, Nagoya, Aichi, 451-0071, Japan. hasebe.yuichi@menard.co.jp

Journal of Dermatological Science
|March 9, 2011
PubMed
Summary

Dermal tissues contain stem cells expressing CD44, CD54, CD90, CD105, and CD271. Specifically, CD54 and CD271 positive stem cells show high proliferation and differentiation potential.

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Area of Science:

  • Stem Cell Biology
  • Dermal Tissue Research
  • Cell Surface Marker Analysis

Background:

  • Mesenchymal stem cells (MSCs) from bone marrow and adipose tissue express markers like CD44, CD54, CD105, CD90, and CD271.
  • These MSCs are known for their pluripotency.
  • The presence and characteristics of stem cells in dermal tissues remain less understood.

Purpose of the Study:

  • To identify and confirm the presence of stem cells within dermal tissues.
  • To evaluate the potential of these dermal stem cells using established MSC markers.
  • To characterize the proliferation and differentiation capabilities of identified dermal stem cell populations.

Main Methods:

  • Immunostaining and Fluorescence-Activated Cell Sorting (FACS) were used to analyze marker expression (CD44, CD54, CD90, CD105, CD271) in neonatal mouse dermis.
  • Cells expressing these markers were isolated.
  • Proliferation potential and differentiation capacity into adipocytes, osteoblasts, and chondrocytes were assessed for isolated cell populations.

Main Results:

  • Stem cells expressing CD44, CD54, CD90, CD105, and CD271 were confirmed in neonatal mouse dermal tissues.
  • Dermal cells positive for CD54 or CD271 demonstrated significant proliferation potential.
  • These CD54 and CD271 positive cells exhibited the ability to differentiate into adipocytes, osteoblasts, and chondrocytes.

Conclusions:

  • Dermal tissues harbor stem cells identifiable by MSC markers CD44, CD54, CD90, CD105, and CD271.
  • Stem cells expressing CD54 and CD271 possess high proliferation and differentiation capacities.
  • These findings suggest dermal stem cells are a viable source for regenerative medicine applications.