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PIMS sequencing extension: a laboratory information management system for DNA sequencing facilities
Peter V Troshin1, Vincent Lg Postis, Denise Ashworth
1College of Life Sciences, University of Dundee, Dundee, DD1 4HN, UK. p.v.troshin@dundee.ac.uk.
BMC Research Notes
|March 10, 2011
Summary
A new software extension for the Protein Information Management System (PIMS) integrates DNA sequencing and liquid handling robots. This system streamlines sample management and data handling for DNA sequencing facilities, improving efficiency and user access.
Area of Science:
- Genomics
- Bioinformatics
- Laboratory Automation
Background:
- DNA sequencing facilities face challenges integrating robotic liquid handling systems with sequencing machines.
- Existing software often lacks seamless integration, hindering overall efficiency.
- A need exists for unified software solutions to manage samples and integrate diverse robotic platforms in sequencing services.
Purpose of the Study:
- To describe an extension of the Protein Information Management System (PIMS) tailored for DNA sequencing facilities.
- To develop a user-friendly web interface for integrated management of the entire sequencing process.
- To enhance sample tracking, data capture, and user communication within sequencing services.
Main Methods:
- Developed a PIMS extension with a web-based interface for DNA sequencing workflows.
- Integrated sample submission, handling, tracking, and data management functionalities.
- Ensured compatibility with specific sequencing machines (Applied Biosystems 3130XL) and liquid handling robots (MWG 4204, Aviso Theonyx).
Main Results:
- The PIMS sequencing extension has been operational since July 2009 at the University of Leeds DNA Sequencing Facility.
- Successfully replaced manual data handling, simplifying data management and user interaction.
- Processed an average of 10,000 samples per month for 45 user groups, demonstrating high throughput and efficiency.
Conclusions:
- The PIMS extension offers a user-friendly, integrated data management solution for DNA sequencing facilities.
- The system facilitates simultaneous access for multiple users and managers via a web browser.
- It integrates robotic systems, manages data flow, and provides remote access to sequencing results, with free availability for academic users.
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