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Transcriptional profiling identifies differentially expressed genes in developing turkey skeletal muscle
Kelly R B Sporer1, Robert J Tempelman, Catherine W Ernst
1Department of Food Science and Human Nutrition, Michigan State University, East Lansing, Michigan 48824, USA.
BMC Genomics
|March 10, 2011
Summary
This study reveals key gene pathways influencing turkey skeletal muscle development and identifies novel genes related to growth and meat quality. Understanding these genetic factors can improve turkey production and meat product quality.
Area of Science:
- Genomics
- Animal Science
- Developmental Biology
Background:
- Skeletal muscle development involves complex gene expression changes crucial for agriculturally important species.
- Intensive genetic selection in turkeys for increased body weight has led to myopathies and meat quality defects.
- A validated turkey skeletal muscle-specific microarray was used to study gene expression.
Purpose of the Study:
- To elucidate functional gene pathways in turkey skeletal muscle development using a microarray.
- To compare gene expression differences between a control turkey line and a line selected for body weight.
Main Methods:
- Skeletal muscle samples were collected from turkeys at three developmental stages (embryo, post-hatch, market age).
- Two turkey genetic lines were analyzed: a randombred control (RBC2) and a selected line (F).
- Microarray hybridizations compared developmental stages within lines and genetic lines within stages.
Main Results:
- 3474 genes showed differential expression related to development (FDR < 0.001).
- 16 genes were differentially expressed between genetic lines (FDR < 0.10).
- Ingenuity Pathways Analysis identified networks, functions, and pathways, with 28 key genes confirmed by qPCR.
Conclusions:
- Novel genes and pathways critical for turkey muscle growth and development were identified.
- Further research will investigate the expression and function of these candidate genes and their protein products.
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