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Updated: Jun 3, 2026

Quantitative Analysis of Cell Edge Dynamics during Cell Spreading
Published on: May 22, 2021
Adhesion patterns in early cell spreading
Pavel Ryzhkov1, Marcus Prass, Meike Gummich
1Institut für Biophysik, Universität Bremen, Bremen, Germany.
Abstract:
Mouse embryonic fibroblasts explore the chemical suitability before spreading on a given substrate. We find this early phase of cell spreading to be characterized by transient adhesion patches with a typical mean size of (1.0 ± 0.4) µm and a lifetime of (33 ± 12) s. Eventually, these patches fuse to initiate extensive spreading of the cell. We monitor cell adhesion using reflection interference contrast and total internal reflection fluorescence microscopy. Digital time lapse movies are analysed employing spatio-temporal correlation functions of adhesion patterns. Correlation length and time can be scaled to obtain a master curve at the fusion point.
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