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Related Experiment Video

Updated: Jun 3, 2026

Sedimentation Equilibrium of a Small Oligomer-forming Membrane Protein: Effect of Histidine Protonation on Pentameric Stability
09:49

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Published on: April 2, 2015

Hydration shells exchange charge with their protein.

H Abitan1, P-A Lindgård, B G Nielsen

  • 1QuP Centre, Department of Physics, Building 309, Technical University of Denmark, DK-2800, Kongens Lyngby, Denmark. Haim.abitan@gmail.com

Journal of Physics. Condensed Matter : an Institute of Physics Journal
|March 10, 2011
PubMed
Summary
This summary is machine-generated.

Ultrasonic pressure waves reveal protein hydration shell dynamics. Proteins and their hydration shells reach equilibrium, which is disrupted by ultrasound and recovers within 45 minutes.

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Area of Science:

  • Biophysics
  • Physical Chemistry
  • Spectroscopy

Background:

  • Understanding protein hydration shells is crucial for protein function.
  • Experimental and theoretical methods for studying protein-hydration shell interactions are challenging.
  • Proteins and their hydration shells exist in a dynamic thermodynamic and charge equilibrium.

Purpose of the Study:

  • To investigate the interaction between proteins and their hydration shells using ultrasonic pressure waves.
  • To explore protein conformational states and hydration shell dynamics under varying pressure.
  • To elucidate the equilibrium dynamics of protein-hydration shell systems.

Main Methods:

  • Utilized ultrasonic pressure waves (0-20 atm) in aqueous protein solutions (β-lactoglobulin, lysozyme).
  • Simultaneously measured Raman spectra to detect spectral changes.
  • Conducted control experiments with amino acids and ethanol solutions.

Main Results:

  • Observed a significant increase (up to 70%) in Raman fluorescence background intensity.
  • Detected changes in vibrational Raman spectra indicating altered protein states.
  • Established a relaxation time of 30-45 minutes for equilibrium recovery after ultrasound application.

Conclusions:

  • Ultrasonic waves disrupt the thermodynamic and charge equilibrium between proteins and their hydration shells.
  • Proteins and hydration shells spontaneously exchange charges in equilibrium.
  • Equilibria are re-established within 30-45 minutes post-ultrasound exposure.