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Related Experiment Video

Updated: Jun 3, 2026

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Published on: March 29, 2019

InTRONs in biotech.

Natasa Skoko¹, Marco Baralle, Sergio Tisminetzky

¹International Centre for Genetic Engineering and Biotechnology, Padriciano 99, 34149 Trieste, Italy.

Molecular Biotechnology

|March 10, 2011

Summary

The spliceosome regulates gene expression through alternative splicing. Introducing introns into sequences can enhance biotechnology applications and molecule production strategies.

Area of Science:

Molecular Biology
Gene Expression Regulation
Biotechnology

Background:

Eukaryotic gene expression involves complex molecular machineries for mRNA maturation, including transcription, processing, export, and translation.
Pre-mRNA splicing, mediated by the spliceosome, joins exons and removes introns, representing a key regulatory mechanism for gene expression.
Alternative splicing allows multiple transcripts from a single gene, contributing to gene expression complexity and regulation.

Purpose of the Study:

To provide an overview of how biotechnology can benefit from intron integration.
To explore the potential of exploiting the splicing process for improved production strategies.

Main Methods:

The study provides a conceptual overview, not detailing specific experimental methods.

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In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

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It focuses on the regulatory role of the spliceosome and alternative splicing in gene expression.

Main Results:

The spliceosome's flexibility enables alternative splicing, a major mechanism for qualitative and quantitative gene expression regulation.
Spliceosome activity influences transcription, mRNA export, stability, and translation, highlighting its broad impact on gene expression.

Conclusions:

The splicing process, particularly alternative splicing, offers significant biotechnological potential for enhancing molecule production.
Integrating introns into sequences of interest can be a valuable strategy for improving biotechnological applications and production processes.