Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 3, 2026

Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence
12:08

Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence

Published on: May 22, 2013

[Quantitative analysis of telomerase reverse transcriptase gene expression in goat reprogramming cells].

Shujin Zhang1, Shuyan Meng, Lei Lei

  • 1Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Veterinary Medicine, Shaanxi Center for Stem Cell Engineering and Technology, Northwest A&F University, Yangling 712100, China.

Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|March 11, 2011
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Unravelling the antifungal mechanism of phytic acid on <i>Aspergillus flavus</i> development and aflatoxin B1 biosynthesis.

Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment·2025
Same author

Efficacy of botulinum neurotoxin-A in the treatment of neuropsychiatric symptoms in patients with Parkinson's disease: an open-label randomized controlled study.

Expert review of neurotherapeutics·2025
Same author

Enhanced Homogeneity in Perovskite Photovoltaic Films via Antisolvent Extraction of a Methylamine-Based Gel Precursor.

ACS applied materials & interfaces·2024
Same author

Attachment characteristics and kinetics of biofilm formation by Staphylococcus aureus on ready-to-eat cooked beef contact surfaces.

Journal of food science·2023
Same author

Methylammonium Chloride as a Double-Edged Sword for Efficient and Stable Perovskite Solar Cells.

Small (Weinheim an der Bergstrasse, Germany)·2023
Same author

Oocyte Arrested at Metaphase II Stage were Derived from Human Pluripotent Stem Cells in vitro.

Stem cell reviews and reports·2023
Same journal

[Recent progress and policy recommendations for biomanufacturing technology in China].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology·2026
Same journal

[CRISPR/Cas9-mediated knockout of the <i>Soc</i> gene in T4 bacteriophage and mutant construction].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology·2026
Same journal

[High-throughput fluorescence-activated droplet sorting of polyethylene terephthalate hydrolases based on fluorescent nanoparticle biosensors].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology·2026
Same journal

[Machine learning-optimized solid-state fermentation of non-grain biomass for enhanced feed protein production].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology·2026
Same journal

[Growth and metabolic characteristics and kinetic modeling of MDCK adherent cells cultured in basket bioreactors].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology·2026
Same journal

[Metabolic engineering of <i>Saccharomyces cerevisiae</i> for <i>de novo</i> biosynthesis of gibberellin A<sub>3</sub>].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology·2026
See all related articles

Telomerase reverse transcriptase (TERT) expression is crucial for induced pluripotent stem cell (iPSC) generation in goats. Higher TERT activity correlates with pluripotency in reprogrammed cells, highlighting its role in cell reprogramming.

Area of Science:

  • Cell Biology
  • Stem Cell Research
  • Gene Expression Analysis

Context:

  • Somatic cell reprogramming into induced pluripotent stem cells (iPSCs) is a key area in cell biology.
  • Understanding the molecular mechanisms driving this process is essential for advancing regenerative medicine.

Purpose:

  • To analyze telomerase reverse transcriptase (TERT) gene expression during goat somatic fibroblast reprogramming.
  • To investigate the relationship between TERT expression and the pluripotency of reprogrammed cells.

Summary:

  • Real-time RT-PCR was used to measure TERT expression in fetal goat tissues and induced goat reprogramming cell clones.
  • TERT expression was significantly higher in testis tissue compared to epithelial tissues.
  • TERT expression was also significantly higher in alkaline phosphatase (AP) staining positive cells (indicating pluripotency) than in AP negative cells.

More Related Videos

A Modified Co-Culture System for Understanding Granulosa-Theca Cell Interactions in the Bovine Ovary
07:03

A Modified Co-Culture System for Understanding Granulosa-Theca Cell Interactions in the Bovine Ovary

Published on: September 19, 2025

Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein
08:26

Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein

Published on: June 12, 2018

Related Experiment Videos

Last Updated: Jun 3, 2026

Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence
12:08

Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence

Published on: May 22, 2013

A Modified Co-Culture System for Understanding Granulosa-Theca Cell Interactions in the Bovine Ovary
07:03

A Modified Co-Culture System for Understanding Granulosa-Theca Cell Interactions in the Bovine Ovary

Published on: September 19, 2025

Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein
08:26

Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein

Published on: June 12, 2018

Impact:

  • The findings indicate that TERT activity plays a critical role in the efficiency and success of cell reprogramming.
  • This research contributes to a deeper understanding of pluripotency induction and may inform future strategies for generating iPSCs in various species.