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Updated: Jun 3, 2026
Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells
Published on: August 9, 2019
Identification of the CD34 enumeration on the day before stem cell harvest that best predicts poor mobilization
David Szwajcer1, Alana Jennings-Coutts, Angeline Giftakis
1Department of Internal Medicine and Pediatrics and Child Health, University of Manitoba and CancerCare Manitoba, Winnipeg, Manitoba, Canada. david.szwajcer@cancercare.mb.ca
Background:
We questioned whether CD34 enumeration in peripheral blood on the day before planned collection would identify a patient population that could benefit from an augmented collection strategy during that mobilization attempt.
Study Design And Methods:
A retrospective review of all adult patients who underwent a first mobilization attempt between January and December 2008 for autologous use was undertaken. Peripheral blood CD34 quantitation on the day before planned collection (Day -1) and day of planned collection (Day 0) was correlated with likelihood of a successful collection.
Results:
Of 41 patients (15 with multiple myeloma, 20 with lymphoma, and six with other malignancies) who underwent mobilization 24 patients (58%) were harvested in 1 day (good mobilizers) with the remaining 17 patients (42%) either requiring more than 1 day to collect or were not collected (poor mobilizers [PMs]). A peripheral blood CD34+ count below 10 × 10(6) /L on Day -1 was optimal in identifying PMs (adjusted odds ratio of 7 [1.4-35]). Increasing the CD34 cutoff from 10 × 10(6) to 15 × 10(6) /L decreased the prediction of poor mobilization (positive likelihood ratio dropped from 3.3 to 2.2).
Conclusion:
Peripheral blood CD34 content of less than or equal to 10 × 10(6) CD34+ cells/L on the day before collection is predictive of poor mobilization whereas higher peripheral blood CD34 counts on Day -1 have a high likelihood of successful 1-day collection.
