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Published on: March 24, 2012
Characterization of proteome alterations in Phanerochaete chrysosporium in response to lead exposure
Volkan Yıldırım1, Servet Ozcan, Dörte Becher
1Department of Biological Sciences, Middle East Technical University, Ankara, Turkey. ozcengiz@metu.edu.tr.
Background:
Total soluble proteome alterations of white rot fungus Phanerochaete chrysosporium in response to different doses (25, 50 and 100 μM) of Pb (II) were characterized by 2DE in combination with MALDI-TOF-MS.
Results:
Dose-dependent molecular response to Pb (II) involved a total of 14 up-regulated and 21 down-regulated proteins. The induction of an isoform of glyceraldehyde 3-phosphate dehydrogenase, alcohol dehydrogenase class V, mRNA splicing factor, ATP-dependent RNA helicase, thioredoxin reductase and actin required a Pb (II) dose of at least 50 μM. Analysis of the proteome dynamics of mid-exponential phase cells of P. chrysosporium subjected to 50 μM lead at exposure time intervals of 1, 2, 4 and 8 h, identified a total of 23 proteins in increased and 67 proteins in decreased amount. Overall, the newly induced/strongly up-regulated proteins involved in (i) amelioration of lipid peroxidation products, (ii) defense against oxidative damage and redox metabolism, (iii) transcription, recombination and DNA repair (iv) a yet unknown function represented by a putative protein.
Conclusion:
The present study implicated the particular role of the elements of DNA repair, post-tanscriptional regulation and heterotrimeric G protein signaling in response to Pb (II) stress as shown for the first time for a basidiomycete.
Insights
White rot fungus Phanerochaete chrysosporium exhibits dose-dependent proteome changes in response to lead (Pb II) exposure. Key proteins involved in DNA repair and oxidative stress response were identified.
Area of Science:
- Environmental Microbiology
- Proteomics
- Biochemistry
Background:
- Investigating the impact of heavy metal toxicity on fungal proteomes.
- Understanding the response mechanisms of white rot fungi to lead (Pb II) exposure.
Purpose of the Study:
- To characterize the total soluble proteome alterations in Phanerochaete chrysosporium exposed to varying doses of Pb (II).
- To identify specific proteins and pathways involved in the fungal response to lead stress.
Main Methods:
- Two-dimensional electrophoresis (2DE) coupled with Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF-MS).
- Analysis of proteome dynamics at different lead concentrations (25, 50, 100 μM) and time points (1, 2, 4, 8 h).
Main Results:
- Dose-dependent response identified 14 up-regulated and 21 down-regulated proteins.
- Specific proteins like glyceraldehyde 3-phosphate dehydrogenase and actin were induced at ≥50 μM Pb (II).
- Proteome dynamics revealed proteins involved in lipid peroxidation amelioration, oxidative stress defense, DNA repair, and transcription.
Conclusions:
- The study highlights the role of DNA repair, post-transcriptional regulation, and G protein signaling in response to Pb (II) stress in basidiomycetes.
- This provides novel insights into the molecular mechanisms of lead tolerance in Phanerochaete chrysosporium.
