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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Jun 3, 2026

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
07:27

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Quantitative miRNA expression analysis using fluidigm microfluidics dynamic arrays.

Jin Sung Jang1, Vernadette A Simon, Rod M Feddersen

  • 1Department of Pulmonary and Critical Care Medicine, Rochester, MN 55905, USA.

BMC Genomics
|March 11, 2011
PubMed
Summary
This summary is machine-generated.

This study introduces a high-throughput microfluidic quantitative PCR (qPCR) platform for microRNA (miRNA) expression profiling. The technology offers a sensitive, reproducible, and cost-effective solution for analyzing miRNA in clinical samples, including formalin-fixed paraffin-embedded tissues.

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Last Updated: Jun 3, 2026

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
07:27

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
10:28

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR
09:26

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR

Published on: January 22, 2014

Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • MicroRNAs (miRNAs) are key gene regulators implicated in human cancers.
  • Existing miRNA detection methods lack high-throughput capabilities for large clinical studies.
  • Formalin-fixed paraffin-embedded (FFPE) samples are crucial for gene expression studies but require efficient analysis methods.

Purpose of the Study:

  • To evaluate a high-throughput qPCR-based microfluidic technology for miRNA expression profiling.
  • To compare this technology with standard qPCR and microarray platforms.
  • To assess the suitability of the technology for analyzing FFPE and fresh-frozen (FF) samples.

Main Methods:

  • Utilized a qPCR-based microfluidic dynamic array system (Fluidigm) for miRNA expression analysis.
  • Compared results with standard singleplex/multiplex qPCR and hybridization-based microarrays.
  • Evaluated miRNA expression in lung cancer cell lines and FFPE/FF clinical samples.

Main Results:

  • Demonstrated high correlation between multiplex and singleplex RT reactions in standard qPCR.
  • Microfluidic technology showed increased sensitivity (left-shifted Ct values) compared to standard qPCR.
  • Reliable detection of miRNAs was achieved with as little as 10 ng of total RNA from both FFPE and FF samples.
  • Observed good overall correlation (R=0.60) between microfluidic qPCR and microarrays, with the microfluidic platform detecting larger fold changes.

Conclusions:

  • The qPCR-array based microfluidic dynamic array platform is suitable for high-throughput miRNA gene expression profiling.
  • This approach is highly reproducible, cost-effective, and uses less sample and reagents than conventional methods.
  • The technology enables rapid, customizable miRNA expression profiling and validation, especially for FFPE samples.