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Related Experiment Video

Updated: Jun 3, 2026

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

Published on: August 4, 2018

Adaptive optics and the eye (super resolution OCT).

D T Miller¹, O P Kocaoglu, Q Wang

¹School of Optometry, Indiana University, Bloomington, IN, USA. dtmiller@indiana.edu

Eye (London, England)

|March 11, 2011

Summary

Adaptive optics Optical coherence tomography (AO-OCT) provides ultrahigh 3D resolution and speed for retinal imaging. This technology visualizes cellular structures in vivo, advancing retinal research and clinical studies.

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Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

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Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

Published on: August 4, 2018

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Published on: January 15, 2013

Area of Science:

Ophthalmology
Biomedical Engineering
Cellular Imaging

Background:

Adaptive optics (AO) combined with optical coherence tomography (OCT) has advanced significantly over the past 8 years.
AO enhances OCT's lateral resolution, reduces speckle, and improves sensitivity, enabling detailed cellular imaging of the retina.

Purpose of the Study:

To showcase the capabilities of AO-OCT for ultrahigh-resolution 3D cellular imaging in the retina.
To present examples of in vivo visualization of retinal structures previously only seen with histology.

Main Methods:

Utilized advanced AO-OCT systems with ultrahigh 3D resolution (3 × 3 × 3 microm³) and high-speed acquisition.
Applied AO-OCT to capture volume images of specific retinal cellular structures.

Main Results:

Demonstrated visualization of individual retinal nerve fiber layer bundles.
Measured diameters of foveal capillaries and the foveal avascular zone.
Quantified spacing and length of cone photoreceptor outer segments near the fovea center.

Conclusions:

AO-OCT provides unprecedented in vivo visualization of retinal microstructures.
This technology is crucial for advancing our understanding of retinal biology and disease.