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Related Concept Videos

Pulmonary Tuberculosis IV01:26

Pulmonary Tuberculosis IV

Tuberculosis, more commonly referred to as TB, is an infectious disease stemming from Mycobacterium tuberculosis. While it primarily impacts the lungs, TB can also affect other body areas. Given its severity and global impact, timely and accurate diagnosis is crucial for controlling its spread and improving patient outcomes.
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
Pulmonary Tuberculosis II01:28

Pulmonary Tuberculosis II

Tuberculosis, or TB, is a bacterial infectious disease caused by Mycobacterium tuberculosis. While its primary impact is on the lungs, leading to pulmonary tuberculosis, it can also affect various other organs, a condition referred to as extrapulmonary tuberculosis.
Here is a detailed explanation of its pathophysiology:
Transmission: The process begins when a person inhales droplet nuclei containing M. tuberculosis. These are typically released into the air when an individual with pulmonary or...
Tuberculosis01:23

Tuberculosis

Tuberculosis (TB) remains a significant global health concern, primarily targeting the lungs and spreading through airborne transmission. Infection begins when aerosolized droplet nuclei, expelled by an individual with active TB, are inhaled by another person. These microscopic particles carry Mycobacterium tuberculosis, the causative agent of TB. Upon reaching the alveoli, the bacilli are engulfed by alveolar macrophages. However, due to their specialized lipid-rich cell wall, these pathogens...
Pulmonary Tuberculosis I01:29

Pulmonary Tuberculosis I

Tuberculosis, often called TB, is a contagious illness primarily caused by Mycobacterium tuberculosis. It mainly affects the lung parenchyma but can also impact other body parts.
Causative Organism
The primary infectious agent causing tuberculosis is Mycobacterium tuberculosis, a slow-growing, acid-fast, aerobic rod that exhibits sensitivity to heat and ultraviolet light. Instances of Mycobacterium bovis and Mycobacterium avium contributing to the development of TB infection are rare.
Mode of...
Pulmonary Tuberculosis III01:31

Pulmonary Tuberculosis III

Tuberculosis (TB) is a contagious infection primarily affecting the lung parenchyma but which can also affect other body parts. TB can be classified based on disease development, presentation, and the affected anatomical site.
The first classification is based on the development of the disease, and it includes the following categories:
Pulmonary Tuberculosis V01:28

Pulmonary Tuberculosis V

Medical management of tuberculosis (TB) patients involves a comprehensive approach that includes diagnosis, treatment, and monitoring. The specific strategies can vary depending on the type of tuberculosis (latent or active), the patient's overall health status, and other considerations.
Latent tuberculosis infection occurs when TB bacteria are present in a person's body, but are not causing illness or symptoms. It is not contagious, and preventive treatment is crucial to avoid the progression...

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Related Experiment Video

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A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening
15:28

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Published on: January 17, 2014

A high-throughput screen for tuberculosis progression.

Ralph Carvalho1, Jan de Sonneville, Oliver W Stockhammer

  • 1ZF-screens B.V., Leiden, The Netherlands. h.p.spaink@biology.leidenuniv.nl

Plos One
|March 11, 2011
PubMed
Summary
This summary is machine-generated.

A new high-throughput zebrafish screening system models tuberculosis progression. This system can study Mycobacterium tuberculosis and identify novel anti-tuberculosis compounds in vivo.

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Area of Science:

  • Microbiology
  • Infectious Diseases
  • Zebrafish Models

Background:

  • Tuberculosis (TB) affects one-third of the global population, with multi-drug resistant strains posing a significant threat.
  • A lack of whole-organism, high-throughput screening systems hinders tuberculosis research and drug development.
  • The zebrafish Mycobacterium marinum infection model mimics human TB pathology, offering biomedical relevance.

Purpose of the Study:

  • To develop a high-throughput screening system for studying tuberculosis progression.
  • To validate the use of zebrafish as a vertebrate model for Mycobacterium tuberculosis infection.
  • To enable high-throughput screening of novel anti-tuberculosis compounds.

Main Methods:

  • Development of a zebrafish infection model using robotic yolk injection for early embryos.
  • Visual flow screening of late-stage larvae for disease progression assessment.
  • Utilizing reverse genetics for high-throughput signal transduction studies.

Main Results:

  • Demonstrated successful propagation of Mycobacterium tuberculosis in zebrafish, mirroring M. marinum infection symptoms.
  • Achieved a throughput of 2,000 embryos per hour, surpassing standard injection methods.
  • Validated the system's efficacy in testing known compounds that prevent TB progression.

Conclusions:

  • The developed zebrafish system provides a novel, high-throughput platform for tuberculosis research.
  • This model system is suitable for studying Mycobacterium tuberculosis and identifying new anti-TB therapeutics.
  • The system facilitates in vivo investigation of disease progression and drug efficacy.