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Updated: Jun 3, 2026

Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli

Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli

Published on: March 16, 2011

Selection of antimetabolite resistant mutants.

¹St.Patrick's College, Kildare, Republic of Ireland.

Methods in Molecular Biology (Clifton, N.J.)

|March 11, 2011

Summary

Plant cell culture mutants resistant to growth-inhibiting chemicals (antimetabolites) are easily selected. These mutants aid research in plant metabolism, genetic manipulation, and improving crop tolerance to diseases and agrochemicals.

Area of Science:

Plant biotechnology
Cellular metabolism
Agrochemicals

Background:

Mutants resistant to antimetabolites are readily selected in plant cell cultures.
Various mutants have been isolated, exhibiting resistance to diverse compounds like amino acid analogs, herbicides, and antibiotics.

Purpose of the Study:

To review progress in the selection of plant cell culture mutants resistant to antimetabolites.
To highlight the utility of these mutants in fundamental research and crop improvement.

Main Methods:

Selection of mutants in plant cell cultures using antimetabolites.
Isolation and characterization of resistant mutants.

Main Results:

Successful isolation of mutants resistant to a wide range of antimetabolites, including amino acid analogs, base analogs, toxins, herbicides, salts, and antibiotics.

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Demonstration of the feasibility of using these mutants for various applications.

Conclusions:

Plant cell culture offers an effective system for selecting antimetabolite-resistant mutants.
These mutants are valuable tools for advancing plant science, genetic engineering, and agricultural practices, particularly in enhancing crop resilience.