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In Vitro Fertilization01:24

In Vitro Fertilization

In vitro fertilization (IVF) is a form of assisted reproductive technology where an egg is fertilized with sperm in a controlled laboratory environment before transferring the resulting embryo into the uterus. This process is designed to help individuals and couples experiencing difficulties conceiving.
The IVF process begins with ovarian stimulation, during which reproductive endocrinologists prescribe hormonal medications to stimulate the ovaries to produce multiple eggs instead of the single...

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Microinjection-based System for In Vivo Implantation of Embryonic Cardiomyocytes in the Avian Embryo
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Implantation of microinjected eggs.

C H Phang1

  • 1Neuropeptide Laboratory, Institute of Molecular and Cell Biology, National University of Singapore, Republic of Singapore.

Methods in Molecular Biology (Clifton, N.J.)
|March 11, 2011
PubMed
Summary

This study details the overnight incubation of microinjected eggs to the two-cell stage for embryo transfer. Surgical techniques for rat oviduct implantation, including precise cutting of the oviduct membrane, are described.

Area of Science:

  • Reproductive biology and developmental science.

Background:

  • Successful embryo transfer requires precise timing and surgical techniques.
  • Mammalian embryo development stages are critical for implantation success.

Purpose of the Study:

  • To describe the standard protocol for incubating microinjected eggs to the two-cell stage.
  • To outline the surgical procedure for embryo implantation into surrogate rats.

Main Methods:

  • Microinjected eggs incubated overnight in M16 culture medium at 37°C in CO₂.
  • Eggs developed to the two-cell stage by 1400 h.
  • Surgical implantation into pseudopregnant rat surrogates, involving a precise incision of the oviduct membrane.

Main Results:

  • Achieved two-cell stage development of microinjected eggs within a defined overnight incubation period.

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  • Demonstrated a reliable surgical method for oviduct implantation in rats, accounting for oviduct membrane toughness.

    • Conclusions:

    • The described incubation and surgical implantation protocol supports successful early-stage embryo transfer in rats.
    • Careful surgical technique is essential for oviduct integrity during embryo transfer procedures in rodents.