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PCR - Polymerase Chain Reaction

PCR - Polymerase Chain Reaction

PCR

PCR

Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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Related Experiment Video

Updated: Jun 3, 2026

Quantification of Adeno-Associated Viral Genomes in Purified Vector Samples by Digital Droplet Polymerase Chain Reaction

Quantification of Adeno-Associated Viral Genomes in Purified Vector Samples by Digital Droplet Polymerase Chain Reaction

Published on: October 11, 2024

Virus genome detection by the polymerase chain reaction (PCR).

J G Hoad¹, T F Schulz

¹Chester Beatty Laboratories, Institute of Cancer Research, London, UK.

Methods in Molecular Biology (Clifton, N.J.)

|March 11, 2011

Summary

The polymerase chain reaction (PCR), developed in 1986, is a vital molecular biology technique. It is now indispensable for detecting virus genomes in both diagnostic and experimental settings.

Area of Science:

Molecular Biology
Virology
Biotechnology

Background:

The polymerase chain reaction (PCR) technique has become a cornerstone of molecular biology since its inception in 1986.
Its versatility has led to widespread adoption across numerous laboratory applications.
PCR is recognized as an essential tool in modern biological research and diagnostics.

Purpose of the Study:

To detail the application of the polymerase chain reaction (PCR) for virus genome detection.
To explore the utility of PCR in both diagnostic and experimental contexts.
To highlight the significance of PCR as an irreplaceable technique in molecular biology.

Main Methods:

The chapter focuses on the principles and application of PCR.
Methodology involves the amplification of nucleic acids to detect viral genetic material.

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Last Updated: Jun 3, 2026

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Specific protocols for diagnostic and experimental virus detection using PCR are discussed.

Main Results:

PCR enables sensitive and specific detection of viral genomes.
The technique is effective for both identifying known viruses and discovering new ones.
Results demonstrate PCR's crucial role in virological studies.

Conclusions:

The polymerase chain reaction (PCR) is an indispensable tool for virus genome detection.
Its application spans critical diagnostic and experimental research in virology.
PCR has revolutionized the field, offering unparalleled capabilities in molecular biology.