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Related Concept Videos

Inhibitors of Virion Maturation and Assembly01:19

Inhibitors of Virion Maturation and Assembly

As part of their replication cycle, certain viruses synthesize long precursor proteins called polyproteins within infected host cells. In human immunodeficiency virus (HIV), two major polyproteins are produced: Gag and Gag-Pol. The Gag polyprotein supplies the structural components of the virus, while Gag-Pol includes essential viral enzymes such as reverse transcriptase, integrase, and protease. After synthesis, these polyproteins move to the host cell membrane, where they assemble into an...
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Antiviral Nucleoside InhibitorsAntiviral nucleoside inhibitors are structural analogs of natural nucleosides that interfere with viral DNA or RNA synthesis. These compounds selectively target viral polymerases due to their resemblance to host nucleosides, thereby disrupting viral genome replication.Mechanism of Acyclovir ActionAcyclovir is a guanosine analog with a three-carbon acyclic side chain. It selectively targets herpes simplex virus type 1 (HSV-1), herpes simplex virus type 2 (HSV-2),...
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Retrovirus Life Cycles

Retroviruses have a single-stranded RNA genome that undergoes a special form of replication. Once the retrovirus has entered the host cell, an enzyme called reverse transcriptase synthesizes double-stranded DNA from the retroviral RNA genome. This DNA copy of the genome is then integrated into the host’s genome inside the nucleus via an enzyme called integrase. Consequently, the retroviral genome is transcribed into RNA whenever the host’s genome is transcribed, allowing the retrovirus to...

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Related Experiment Video

Updated: Jun 3, 2026

Rapid Screening of HIV Reverse Transcriptase and Integrase Inhibitors
05:46

Rapid Screening of HIV Reverse Transcriptase and Integrase Inhibitors

Published on: April 9, 2014

Assessment of Compounds for Anti-HIV Activity.

C Patience1, J Moore, M Boyd

  • 1Chester Beatty Laboratories, Institute of Cancer Research, London, UK.

Methods in Molecular Biology (Clifton, N.J.)
|March 11, 2011
PubMed
Summary

Researchers are developing assays to find antiviral compounds that inhibit human immunodeficiency virus (HIV) replication. These methods assess compound effectiveness by measuring viral presence in cell cultures, aiding in the search for AIDS treatments.

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Last Updated: Jun 3, 2026

Rapid Screening of HIV Reverse Transcriptase and Integrase Inhibitors
05:46

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Published on: April 9, 2014

Early Viral Entry Assays for the Identification and Evaluation of Antiviral Compounds
09:29

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Assays for the Identification of Novel Antivirals against Bluetongue Virus
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Published on: October 11, 2013

Area of Science:

  • Virology and Immunology
  • Drug Discovery and Development

Background:

  • Human immunodeficiency virus (HIV) is the causative agent of acquired immunodeficiency syndrome (AIDS).
  • Significant research efforts are dedicated to discovering antiviral compounds against HIV.
  • CD4 T-cell lines are commonly used in assays due to CD4's role as the primary HIV receptor.

Purpose of the Study:

  • To outline the principles and methodologies for assessing antiviral activity against HIV.
  • To highlight the utility of various assay systems in evaluating potential anti-HIV compounds.

Main Methods:

  • Utilizing established cell culture systems, often involving CD4+ T-cell lines.
  • Exposing the virus to test compounds under controlled laboratory conditions.
  • Measuring key parameters indicative of viral replication and inhibition.

Main Results:

  • Assay systems provide a convenient and effective means to screen for antiviral properties.
  • The described methods allow for the quantitative assessment of compound efficacy against HIV.

Conclusions:

  • The development and application of robust assay systems are crucial for identifying novel anti-HIV therapeutics.
  • These assays facilitate the ongoing search for effective treatments to combat HIV/AIDS.