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Updated: Jun 3, 2026

Laboratory Scale Production and Purification of a Therapeutic Antibody

Laboratory Scale Production and Purification of a Therapeutic Antibody

Published on: January 24, 2017

Production and Purification of Hybrid Ty-VLPs.

N R Burns¹, J E Gilmour, S M Kingsman

¹British Bio-technology Ltd., Oxford, UK.

Methods in Molecular Biology (Clifton, N.J.)

|March 11, 2011

Summary

Researchers engineered hybrid virus-like particles (VLPs) using yeast Ty element proteins. These novel Ty-VLPs can incorporate various foreign proteins, demonstrating a versatile platform for biotechnological applications.

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Area of Science:

Biotechnology and Molecular Biology
Virology and Protein Engineering

Background:

The Ty element of yeast encodes a protein with self-assembly properties.
This self-assembly capability can be utilized for creating novel particulate structures.

Purpose of the Study:

To develop a method for producing hybrid Ty virus-like particles (VLPs).
To demonstrate the incorporation of diverse foreign protein sequences into Ty-VLPs.

Main Methods:

Development of expression vectors for constructing Ty fusion genes.
Fusion of protein-coding sequences of interest with the Ty gene.
Production and characterization of hybrid Ty-VLPs.

Main Results:

Successfully produced various hybrid Ty-VLPs carrying foreign proteins.
Incorporated proteins ranged from 3 to 42 kDa.
Examples include proteins from human immunodeficiency virus-1 (HIV-1), influenza virus, and bovine papillomavirus.

Conclusions:

The Ty VLP system provides a flexible platform for producing hybrid particles.
This technology enables the display of diverse functional protein domains.
Potential applications in vaccine development, drug delivery, and protein research.