Related Experiment Video
Updated: Jun 3, 2026
Real-Time Polymerase Chain Reaction-Based Detection and Quantification of Hepatitis B Virus DNA
Published on: December 15, 2023
Hepatitis B virus : detection and quantitation by membrane and liquid hybridization, branched DNA signal
1the Toronto Hospital, Toronto, Ontario, Canada.
Abstract:
Hepatitis B virus (HBV) is a member of the Hepadnaviridae family and has a (3200-bp) partially double-stranded circular DNA genome (1). This virus causes subclinical, acute self-limited, chronic, and fulminant hepatic infections. Chronic HBV infection is particularly important because of the clinical manifestations which include: cirrhosis, hepatic failure, hepatocellular carcinoma, as well as extrahepatic manifestations, such as vasculitis and membranoproliferative glomerulonephritis. It is estimated that there are currently 350 million chronic HBV carriers worldwide (2).
